Κατεύθυνση Ανόργανη Χημεία και Εφαρμογές της στη Βιομηχανία
Library of the School of Science

2024-06-27

2024

Karampatzaki Lamprini-Anna

Ελένη Ευθυμιάδου, Αναπληρώτρια Καθηγήτρια Τμήμα Χημείας ΕΚΠΑ (Επιβλέπουσα),
Κωνσταντίνος Μεθενίτης, Αναπληρωτής Καθηγητής Τμήμα Χημείας ΕΚΠΑ,
Ευαγγελία Χρονοπούλου, Επικ. Καθηγήτρια Γεωπ/κο Πανεπιστήμιο Αθηνών

Σύνθεση, χαρακτηρισμός και βιολογική αξιολόγηση νανοσωματιδίων σιδήρου επικαλυμμένα με πολυσακχαρίτες

Greek

Synthesis, characterization and biological evaluation of iron nanoparticles coated with polysaccharides

In recent years, due to the development of nanotechnology, magnetic nanoparticles have become increasingly important to the scientific community. Iron oxide nanoparticles are the most extensively studied systems. Their widespread use is due to a number of important characteristics such as non-toxicity, biocompatibility and magnetic properties. Particular attention is paid to their biological applications, in particular in the fight against cancer.
In this thesis, the synthesis, characterization and biological evaluation of magnetic iron oxide nanoparticles were carried out. The synthetic method followed is that of co-immersion. Coatings with citrate, APTES and with the polysaccharides HPC and levan were carried out in order to improve the biocompatibility and colloidal stability of the nanoparticles.
The structural and magnetic characterization of the above compositions was carried out using infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV-vis), dynamic light scattering (DLS), powder X-ray diffraction (PXRD), transmission electron microscopy (TEM) and magnetic superheating. This was followed by quantification of the iron concentration and nanoparticle coatings. Biological evaluation was performed on the healthy cell line HaCaT and cancer cell line U87MG and the bare and polysaccharide-coated nanoparticles were studied. The methods followed were MTT method, Prussian blue assay, TMB method and hematological compatibility study.
From the results it is concluded that the nanoparticle coatings were successfully carried out by affecting the structure, colloidal stability and magnetic properties. From a biological point of view, all the synthesized nanoparticles showed a satisfactory degree of biocompatibility

Science

Iron, Iron oxide, Polysaccharide, Magnetic nanoparticles, Cytotoxicity

Yes

10

Yes

84

126

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https://pergamos.lib.uoa.gr/uoa/dl/object/3401958