Unit:
Κατεύθυνση Ανόργανη Χημεία και Εφαρμογές της στη ΒιομηχανίαLibrary of the School of Science
Author:
Ravanou Anastasia
Supervisors info:
Κωνσταντίνος Μεθενίτης, Αναπληρωτής Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Original Title:
Synthesis and characterization of a Cu(II) complex with a curcumin derivative. Study of the interaction with CT-DNA, BSA and chitosan-based aerogels.
Translated title:
Synthesis and characterization of a Cu(II) complex with a curcumin derivative. Study of the interaction with CT-DNA, BSA and chitosan-based aerogels.
Summary:
In the present work, the synthesis and characterization of a copper(II) complex with a curcumin derivative was studied. The interaction of that complex (Cu-curc) with CT-DNA (Calf Thymus DNA) and BSA (Bovine Serum Albumin) protein as well as its in vitro biological activity as a potential new anti-cancer drug were investigated. The choice of the ligand (curc) was based on the interaction of curcumin and its various derivatives with DNA and proteins of organisms, where they show interesting biological activity as potential therapeutic agents, inducing apoptosis in cancer cells and death in unwanted microorganisms or viruses. Complexation with transition metals enhances this activity. The structure of curc and the successful complexation with Cu(II) were confirmed via the characterization of both curc and Cu-curc using several techniques (ATR FTIR, NMR, fluorescence spectroscopy, mass spectrometry and UV-Vis). In addition, the interaction of Cu-curc with CT-DNA and BSA protein was investigated using cyclic dichroism (CD), UV-Vis spectroscopy, fluorescence spectroscopy and the hydrodynamic method of viscometry. The results showed that Cu-curc interacted strongly with DNA. Then, Cu-curc was bound to BSA protein strongly, which enabled its transport in the blood. The potential of both curc and Cu-curc to interact with novel chitosan-based aerogels was also investigated using fluorescence spectroscopy.
Main subject category:
Science
Keywords:
aerogels, anticancer activity, curcumin, BSA (Bovine serum albumin), chitosan, copper, CT-DNA (Calf Thymus DNA), transition metal-complex.
Number of references:
137
File:
File access is restricted until 2027-10-29.
RESEARCH DIPLOMA THESIS ANASTASIA RAVANOU FINAL.pdf
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File access is restricted until 2027-10-29.
