Summary:
During the course of this study we focused on:
The thorough characterization of the bone marrow Mesenchymal Stem Cells MSC
(BM-MSC) population derived from the BALB/c species, considering the
significance of the murine model amongst animal models. Specifically, we
examined the effect of gender, age, and in vitro culture on the basic
properties (proliferation, differentiation, and immunosuppressive potential) of
mouse BM-MSC (mBM-MSC); and found a decline of the progenitor frequencies in
the BM with age, lower MSC frequencies in all female donors, and an increase in
the mBM-MSC proliferation rate upon in vitro propagation.We report, for the
first time, the expression of Crdbp/Imp1 and Dazl in mBM-MSC. Dazl, Oct3/4, and
Sox2 were detected in relatively low levels in contrast to Crd-bp/Imp1, its
major target c-Myc, as well as Nanog, which were expressed redundantly,
irrespective of sex, donor age, or in vitro passaging.
The expression of CRD-BP/IMP1 in different MSC populations of human origin,
specifically BM-MSC, adipose tissue (AT), and umbilical cord (UC). Here we
demonstrate for the first time that MSC have a differential pattern of
CRD-BP/IMP-1 expression that could correlate with their proliferation rate.
Members of the TET family of demethylases are involved in the epigenetic
regulation of CRD-BP/IMP-1 in human MSC.
Determining the effect of intravenous administration of young mBM-MSC to
elderly mice in the alleviation of the aging phenotype in a preclinical mouse
model, as aging has been recently linked to stem cell exhaustion. Here we show
the positive outcome of this administration.
Keywords:
Mesenchymal stem cells, Ageing, TET proteins, Embryonic proteins, IGF2BP1
