Τομέας Παθολογίας
Library of the School of Health Sciences

2014-12-19

2014

Κουρής Ανάργυρος

Κατούλης Αλέξανδρος, Γιαμαρέλλος-Μπουρμπούλης Ευάγγελος, Σταυριανέας Νικόλαος

Ψωρίαση. Διερευνηση μεταβολών των Τ-λεμφοκυττάρων στην ανοσολογική διαταραχή των ασθενών με ψωρίαση

Greek

Psoriasis. Investigation of T-lemphocytes in immunological disorders of patients with psoriasis

Psoriasis is one of the most common immune-mediated chronic inflammatory skin
diseases, in which proinflammatory cytokines, such as, TNF-α, IL-17, IL-1β,
IL-6 and IL-22 play an important pathogenetic role.-The aim of this study is to
assess whether proinflammatory cytokines TNF-α, IL-17, IL-1β, IL-6 and IL-22
are released systemically during psoriasis, arguing for a generalized
inflammatory reaction. Peripheral blood mononuclear cells (PBMCs) were isolated
from 30 patients with psoriasis and from 30 healthy volunteers. Cytokine
production was assessed in supernatants after stimulation of PBMCs with
microbial stimuli by an enzyme immunoassay. In addition, flow cytometry was
used to determine the subsets of monocytes involved and-the intracellular TNF-α
production in monocytes. IL-17 levels were-significantly higher in supernatants
of PBMCs of psoriatic patients compared to controls after stimulation with PHA
(p= 0.042).TNF-α production was-significantly higher in cells of psoriatic
patients compared to controls after-stimulation with all stimuli.-A
statistically significant difference was observed between-patients and controls
for CD14+/CD16+ monocytes (p<0.0001) and CD14-/CD16+ monocytes-(p=0.002).
TNF-α produced by monocytes and the absolute counts of CD14-/CD16+ monocytes
were greater in psoriatic patients compared to healthy controls.-There is
evidence that this subpopulation may be responsible for the increased TNF-α
production by monocytes.

Psoriasis, TNF-a, Monocytes

No

0

No

295

111

File access is restricted only to the intranet of UoA.

https://pergamos.lib.uoa.gr/uoa/dl/object/1308428