Unit:
Τομέας ΙΙ [Οργανική Χημεία – Οργανική Χημική Τεχνολογία – Χημεία Τροφίμων]Library of the School of Science
Author:
Σταματάκης Γεώργιος
Dissertation committee:
Κωνσταντίνος. Α. Δημόπουλος, Καθηγητής ΕΚΠΑ (επιβλέπων) Σμαραγδή Αντωνοπούλου, Καθηγήτρια Χαροκόπειου Πανεπιστημίου, Δέσποινα Περρέα, Καθηγήτρια ΕΚΠΑ
Original Title:
Βιομόρια από απόβλητα Ελαιουργίας: Μελέτη δομής και in vitro και in vivo δράσεις αυτών
Translated title:
Biomolecules from olive wastes: structure elucidation and study of their in vitro and in vivo effects
Summary:
The present study investigated the existence and structure of biologically
active lipids in Olive Mill Waste (OMW), and studied the anti-atherogenic
actions of a new functional food (yogurt 2 %, PY), enriched with extract from
the OMW in rabbits. The biologically active lipids extracted from OMW were
separated by HPLC. The collected fractions were tested in washed rabbit
platelets and the most potent was characterized by mass spectrometry. For the
in vivo study of the inhibition of atherosclerotic plaques rabbits were fed for
48 days on an atherogenic diet while co administering PY enriched with 500mg
OMW/120g yoghurt and PY in 2 separate groups. For the regression of
atherosclerotic plaques animals were fed the atherogenic diet for 45 days and
a) standard rabbit food (RF) and PY with OMW and b ) RF and PY while both
interventions had their control groups. The animals were euthanized and the
plaques were morphometric assessed along with biochemical/enzymatic assays. In
the inhibition groups animals fed with PY and OMW showed a decrease in the
plaques thickness compared with the control group which is consistent with the
reduction of IL-1b. Regarding the regression of atherosclerotic plaques, yogurt
administration, plain or enriched, lowered blood lipids (total and LDL
cholesterol , triacylglycerols) to basal levels . Atherosclerotic plaques
significantly decreased in the group fed with enriched yogurt, which was again
associated with the reduction of IL-1b compared with the control group and the
PY group. Lp-PLA2 increased in all groups with the uptake of atherogenic diet,
but dropped below basal levels after the yoghurt intake. PAF metabolic enzymes,
in cells and tissues, in both interventions differentiated in a way consistent
with the favorable results from the use of yogurt plain or enriched.
Keywords:
Atherosclerosis, PAF, Olive mill wastes, Yoghurt, Rabbit
Number of index pages:
1-10
Number of references:
502
