Τομέας Γενετικής και Βιοτεχνολογίας
Library of the School of Science

2014-06-10

2014

Παπαϊωάννου Ιωάννης

Καθηγητής Μιλτιάδης Α. Τύπας, Καθηγητής Επαμεινώνδας Ι. Παπλωματάς, Επίκουρη Καθηγήτρια Αικατερίνη-Μαρία Παππά

Γενετική και μοριακή μελέτη της ετεροκαρύωσης στον φυτοπαθογόνο μύκητα Verticillium

Greek

Genetic and molecular study of heterokaryosis in the plant pathogenic fungus Verticillium

Anastomosis of conidia or hyphae is the first step of the parasexual cycle in
ascomycetes. This process, however, is often hampered in natural populations of
fungi by vegetative or heterokaryon incompatibility systems. Based on their
compatibility behavior, strains of the asexual phytopathogenic ascomycetes of
the genus Verticillium are classified to Vegetative Compatibility Groups
(VCGs), which are traditionally regarded as genetically homogeneous and
isolated subspecific groups. The goal of this work was to study
heterokaryosis/heterokaryon incompatibility mainly in V. dahliae with
population, genetic, cell biology, genomic and molecular-functional
experimental approaches. With these methods, (i) new methods were developed for
the high-throughput VCG classification of fungal populations and the rapid
molecular prediction of classification, (ii) it was shown that V. dahliae VCGs
are not genetically homogeneous and absolute genetic barriers are absent
between VCGs; a marked impact of the genetic markers used on the VCG
classification of strains was also observed, (iii) anastomoses of conidia, the
establishment of heterokaryons and the incompatibility phenotype were studied
at the microscopic level, (iv) the putative heterokaryon
incompatibility-related gene repertoire of Verticillium species were identified
and analyzed in silico, and the selected genes het-c1, het-c2, un-24, vib-1,
hex-1 and MAT1-2-1 were functionally examined through the construction of gene
knock-out deletion mutants; several phenotypic alterations both in the
compatibility behavior and fundamental morphogenetic/physiological processes
were recorded.

Hyphal/conidial anastomosis, Het genes, Heterokaryosis, Vegetative compatibility groups, Nuclear ribosomal DNA

Yes

vi-xi, xvii-xxiii

Yes

515

xxiv, 264, VII

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https://pergamos.lib.uoa.gr/uoa/dl/object/1308780