Unit:
Διατμηματικό / Διϊδρυτικό ΠΜΣ Μοριακή ΙατρικήLibrary of the School of Health Sciences
Supervisors info:
Αναπληρωτής Καθηγητής Αντώνης Σταματάκης, Ρεβέκκα Μάτσα, Δήμητρα Θωμαΐδου
Original Title:
Κατασκευή πλασμιδιακού φορέα έκφρασης της RanBPM για in vivo λειτουργικές μελέτες της αλληλεπίδρασης των πρωτεϊνών RanBPM και BM88/Cend1 κατά τη νευρογένεση
Translated title:
Construction of expression plasmid vector of RanBPM for in vivo functional studies of the interaction of the proteins RanBPM and BM88 / Cend1 during neurogenesis
Summary:
During development, coordinate regulation of cell cycle and differentiation of
neural progenitor cells is essential for the production of adequate numbers and
types of mature cells. One of the molecules involved in this process is RanBPM,
a scaffolding protein expressed in the immune and nervous system (Murrin et al
2007). Previous in vitro studies have shown that RanBPM interacts with protein
BM88 / Cend1 (Tsioras et al 2013). Cend1 is a neuro-specific protein involved
in the output of both neural stem / progenitor cells from the cell cycle and
neuronal differentiation (Koutmani et al 2004, Politis et al 2007, Sergaki et
al 2010). The purpose of this research project was to construct a plasmid
expression vector of RanBMP for in vivo functional studies of RanBMP and Cend1
during neurogenesis. First of all, a plasmid vector that co-expresses RanBPM
with a fluorescent protein was constructed. The verification of the
functionality of the recombinant plasmid vector was performed with transfection
experiments in HEK293T cell line and the overexpression of this molecule was
tested by immunoblotting and immunofluorescence staining. Eventually, the
transfection of mouse fetal brain (E 14.5) with plasmid vectors that
overexpress proteins RanBMP and Cend1 has followed. In conclusion, in this
paper are presented the first results of the in vivo interaction of proteins
RanBMP and Cend1 and their role in neurogenesis.
Keywords:
RanBPM, Cend1, Neurogenesis, Fetal brain, Plasmid
Number of references:
122
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