Unit:
ΠΜΣ Μικροβιακή ΒιοτεχνολογίαLibrary of the School of Science
Author:
Μωυσή Κωνσταντίνα
Supervisors info:
Αμαλία Καραγκούνη Καθηγήτρια
Original Title:
Διερεύνηση της δυνατότητας παραγωγής λιπιδίων κατάλληλων για βιοντίζελ από ενδογενή στελέχη κυανοβακτηρίων και μικροφυκών του ελλαδικού χώρου
Translated title:
Investigation of the productivity of endogenous cyanobacyteria and microalgae in lipids suitable for biodiesel from greek environments
Summary:
The rase of the price of fossil fuels and of the emission of Greenhouse gasses
has led to the need of biofuel production. First generation biofuels have the
significant advantage that they can be used in the present internal combustion
engines. But they have the basic disadvantage that they compet the food
industry, as they are extracted from oil crops and vast lands are needed for
their growth. Second generation biofuels were then developed. These biofuels
are extracted from energy crops but the handling of these crops are still under
development. One of the biofuels that is used in EU is biodiesel. The
scientific community tried to find low cost sources for biofuel production and
they discovered that microalgae and cyanobacteria are two prommising
candidates. The purpose of this study was to isolate microalgae and
cyanobacteria from Greek environments, grow them in open, closed and fed batch
cultures and the selection of the most productive strains. &4 microalgae and 4
cyanobacteria were isolated from the monuments of Acropolis, lake Koroneia and
the region of Aspropirgos and were phenotypically and molecularly identified.
All strains were grown in closed liquid cultures and the strains with the
highest biomass production were selected and grown in fed batch cultures.The
lipid productivity was estimated by Flow Cytometry and Gas Chromatography. The
strains Chlorella sp. AKA17 and ASP14 were selected as they presented the
highest biomass and lipid productivity, the optimum nitrogen concentration and
pH value for each strain was estimated and their cultures were scaled up to 15
l. Finally the strain Chlorella sp. AKA17 was selected as the most lipid
productive.
Keywords:
Microalgae, Cyanobacteria, Biodiesel, Lipids, Isolation
Number of references:
157
