Κατεύθυνση Κλινική Χημεία
Library of the School of Science

2015-10-15

2015

Αγγελίδης Ηλίας

Ε. Λιανίδου Καθηγήτρια

Μελέτη της έκφρασης του υποδοχέα των ανδρογόνων και των εναλλακτικών του μεταγράφων σε κυκλοφορούντα καρκινικά κύτταρα στον καρκίνο του προστάτη

Greek

Study of the expression of the androgen receptor and its splice variants in circulating tumor cells in prostate cancer

Metastasis, the spread of tumor cells (Circulating Tumor Cells, CTCs) from the
primary tumor to distant organs and the development of secondary outbreaks, is
one of the most dangerous aspects of cancer. Thus the molecular
characterization of CTCs, may assist in the prognosis of cancer, in the design
of new therapeutic drugs and finally in the understanding of the biology of
metastasis. Due to the fact that the number of cells isolated from the
peripheral blood of the patients and the extracted RNA available for analysis
is limited, the development of new technologies that promote the simultaneous
detection of the expression of many genes, is essential.
Prostate cancer, the topic of this thesis, is the most common malignancy in men
a fact that justifies the rising number of biomarkers that have been studied in
CTCs so as to correlate the clinical characteristics of patients with their
clinical outcome. An ideal biomarker should be specific for the cancer cells
studied, and should indicate the specific tissue of origin. A biomarker must
not appear in any other cell type of normal tissue and must also be easily
detectable with low dispersion and high clinical relevance.
The objective of this study is to assess the diagnostic sensitivity and
specificity of three protocols for the quantification of 6 different genes
(B2M, PCGEM1 AR1, AR3, AR567es, ARTotal) using the polymerase chain reaction
technology. The latter 4 genes where studied through a tetraplex Real Time PCR
assay the development of which demanded careful in- silico design of the
primers and of the four different double hybridization probes needed. These
probes where labeled with four different dyes that differ in their fluorescence
emission spectra. The method was developed in the LightCycler 2.0 instrument
since it includes six detection channels.
The developed methods where applied on 16 samples derived from normal blood
donors and 69 samples from patients with prostate cancer whose CTCs were
isolated by the Ficoll method. In most cases (> 75%) patients were metastatic.
Over 30% of the patients seem to express at least one of the AR’s Splice
Variants a fact that places yet again the androgen receptor in the epicenter of
the research on the prostate’s malignancy.

B2M, PCGEM1, AR Splice Variants, multiplex-PCR, CTCs

Yes

1-10

Yes

175

152

File access is restricted.

https://pergamos.lib.uoa.gr/uoa/dl/object/1318642