Summary:
In the present Master thesis four African propolis samples were studied, three
samples from Cameroon and one sample from Congo that was analyzed for the first
time. Through chromatographic procedures 21 secondary metabolites were isolated
(14triterpenoids, 2 monoterpenic alcohols, 4diprenyl flavonoids and 1 fatty
acid ester), 11 for the first time in propolis (lonchocarpol-A,
6,8-diprenyl-eriodictyol, 6,8-diprenyl-aromadendrin, lespendezaflavonone-C ψ-
taraxasterol-acetate, taraxasterol-acetate, 3α-hydroxy-olean-12-en-30ol,
betuline-aldehyde , bachara-12,21-dien-3β-ol, erythrodiol, 1,8-terpineol).
Subsequently the RI indices were found for the pure terpenic constituents and
through GC-MS analyses in the ethanol extracts of each sample 4 additional
triterpenoids and 2 monoterpenic alcohols were identified. Total Polyphenol
Content (TPC) was estimated for each sample by Folin-Ciocalteu method with
that of Congo propolis and one propolis from Cameroon to be higher. Finally,
all extracts were studied for their antimicrobial action against 6 bacteria and
3 pathogenic fungi by dilution method and was found that Congo propolis and
one sample from Cameroon to show higher action due to their higher
concentration in phenolic compounds. Finally it should be noted that the
intence presence of triterpenoids and prenylflavonoids in these samples agrees
with the studies published so far for the African propolis.
Keywords:
Propolis, Triterpenoids, Diprenyl-flavonoids, Folin Ciocalteu, RI Indices
