Investigation of the role of RANKL gene in the Pathogenesis of Pulmonary Fibrosis

Postgraduate Thesis uoadl:1337720 629 Read counter

Unit:
Κατεύθυνση Κλινική Χημεία
Library of the School of Science
Deposit date:
2017-03-16
Year:
2017
Author:
Sompatzi Eleni
Supervisors info:
Αϊδίνης Βασίλης: Ερευνητής Α’ στο Ερευνητικό Κέντρο Βιοϊατρικών Επιστημών (ΕΚΕΒΕ) «Αλέξανδρος Φλέμινγκ».
Χρήστος Κρούπης: Επίκουρος Καθηγητής Κλινικής Βιοχημείας - Μοριακής Βιολογίας, Π.Γ.Ν. «Αττικόν», Ιατρική Σχολή, Πανεπιστήμιο Αθηνών.
Ευρύκλεια Λιανίδου: Καθηγήτρια Αναλυτικής Χημείας, Σχολή Θετικών Επιστημών, Τμήμα Χημείας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών.
Original Title:
Μελέτη του ρόλου του γονιδίου RANKL στην Παθογένεια της Πνευμονικής Ίνωσης
Languages:
Greek
Translated title:
Investigation of the role of RANKL gene in the Pathogenesis of Pulmonary Fibrosis
Summary:
Idiopathic pulmonary fibrosis (IPF) is a fatal disease of unknown etiology. The main features of the disease is the increased collagen deposition in the lung, which is normally produced in response to wound healing. In order to analyse the caracteristics of IPF we used the bleomycin induced animal model. This complicated disease involving many different biological pathways that can affect the mechanism and progression . Recently found the expression of RANKL / RANK / OPG in specific tissue cells with pulmonary fibrosis and the role of RANKL / OPG ratio in the disease.
The main role of these molecules is bone metabolism. RANKL (receptor activator of NF-kB ligand) controls the remodeling of bone, regulating the differentiation, maturation and survival of osteoclasts, which are responsible for bone resorption. RANK known as RANKL signaling receptor (receptor activator of NF-kB) participates in the formation of osteoclasts. The OPG as a soluble decoy receptor of RANKL,competes with RANK for RANKL binding,thereby blocking RANK activation.
According to these data, we focused on verifying the expression of these molecules in tissues of mice with pulmonary fibrosis and explore the role of genes RANKL / RANK / OPG in the pathogenesis of the disease. For this reason we challenged IPF in TghuRANKL and C57 / Bl6 mice by intratracheal injection of bleomycin, to evaluate the expression levels of these genes and especially to understand if they work positively or negatively in the development of the disease in both genotypes.
In conclusion ,based on the experimental results, there is no difference in the pathogenesis of pulmonary fibrosis in both genotypes. Key role in RANKL action plays the RANKL / OPG ratio.
Main subject category:
Science
Other subject categories:
Health Sciences
Keywords:
Idiopathic pulmonary fibrosis RANKL, RANK, OPG, TghuRANKL.
Index:
No
Number of index pages:
0
Contains images:
No
Number of references:
55
Number of pages:
135
ΜΕΛΕΤΗ ΤΟΥ ΡΟΛΟΥ ΤΟΥ ΓΟΝΙΔΙΟΥ RANKL ΣΤΗΝ ΠΑΘΟΓΕΝΕΙΑ ΤΗΣ ΠΝΕΥΜΟΝΙΚΗΣ ΙΝΩΣΗΣ.pdf (3 MB) Open in new window