Unit:
Τομέας ΧειρουργικήςLibrary of the School of Health Sciences
Author:
Patsaki Anastasia
Dissertation committee:
1) Ισμήνη Δοντά, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ.
2) Σοφία Μητάκου, Καθηγήτρια, Φαρμακευτική Σχολή, ΕΚΠΑ
3) Αλέξιος-Λέανδρος Σκαλτσούνης,Καθηγητής, Φαρμακευτική Σχολή, ΕΚΠΑ
4) Νικόλαος Παπαιωάννου, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
5) Αγγελική Τριανταφύλλου-Πιτίδου, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
6) Ευα Κασσή, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
7) Ιωάννης Τριανταφυλλόπουλος, Επίκουρος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Μελέτη της δράσης του εκχυλίσματος Αmphimas pterocarpoides στην πρόληψη της οστεοπόρωσης σε πειραματικό πρότυπο θήλεος ωοθηκεκτομημένου επίμυος.
Translated title:
Evaluation of Amphimas pterocarpoides plant extract on the prevention of osteoporosis in estrogen deficient ovariectomized rats
Summary:
Ιntroduction: Osteoporosis is a major health threat and its prevalence is expected to rise dramatically in the coming decades. Hormone replacement therapy (HRT) plays a crucial role in the management and the prevention of osteoporosis, but prolonged use has been associated with adverse effects such as breast and uterine cancer, thromboembolic episodes and coronary heart disease. This has led to an increased demand for alternative therapies. In the present study, we assessed the phytoestrogenic potential of Amphimas pterocarpoides (AP) plant extract on the ovariectomy induced bone loss in rats in two different doses.
Methods and materials: Fifty five female (10 month old) Wistar rats were used. Ovariectomy (OVX) was performed in 44 rats, while sham operation was performed in the remaining 11 rats (Control group). Ovariectomized animals were separated in the OVX group (n=13) and received drinking water ad libitum right after the surgery until the end of the experiment, while the treated groups received Amphimas pterocarpoides extract at 50 mg/kg (ALow group, n=15) and at 150 mg/kg (AHigh group, n=16). Measurements of Bone Mineral Density (BMD) were established using dual-energy X-ray absorptiometry (DEXA) and performed at baseline, 3 and 6 months after ovariectomy. After 6 months treatment, the rats were euthanized, and their femurs were extracted. The femoral biomechanical properties were evaluated using the 3-point bending test (3PB).
Results: The percentage changes of the mean values of the proximal tibia BMD from baseline to 3 months for Control, OVX, ALow and AHigh, were 6.254%, -21.822%, -14.512% and -4.421% whereas, the percentage changes from baseline to 6 months were 8.108%, -32.134%, -23.985% and -8.144 accordingly. The 3PB test revealed that the maximal load before fracture (Fmax) demonstrated a significant higher value in the ALow group compare to the OVX group (112.69 Mpa vs. 96.9 Mpa, p<0.001). In addition the mean values for Stress in the OVX group was significant lower in contrast to ALow group (158.48 Mpa vs. 180.5 Mpa, p=0.011). AHigh group presented higher values in Fmax and Stress in contrast to the OVX group, but statistical significant values did not observed between groups.
Conclusions: This study suggests that orally administered Amphimas pterocarpoides for 6 months, manage to prevent the rapid degradation ofbone and is effective in terms of improving both bone quantity (bone density) and quality (biomechanical parameters), surrogate markers of reduced risk fracture risk.
Main subject category:
Health Sciences
Keywords:
Osteoporosis, Phytoestrogens, Bone mineral density, Ovariectomy, Biomechanical properties
Number of references:
297
