Supervisors info:
Ράλλης Μιχαήλ, Επίκουρος Καθηγητής Τομέα Φαρμακευτικής Τεχνολογίας, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Παρασκευάς Δάλλας, Επίκουρος Καθηγητής Τομέα Φαρμακευτικής Τεχνολογίας, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Βλάχου Κωνσταντινίδου Μαριλένα, Επίκουρη Καθηγήτρια Τομέα Φαρμακευτικής Τεχνολογίας, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Summary:
Exposure to ultraviolet radiation is a daily phenomenon for every human being and can cause a variety of positive or negative effects on the skin, as a function of the dose that falls on it. Many studies have been associated with the enhancement of free radical production and, consequently, with the increase of oxidative stress in the body. As a result, several pathological conditions of the skin, such as premature aging, sunburn, and inflammation in general occur.
To reduce the harmful effects caused by oxidative stress, the body needs a complex system of protection: the antioxidant system. This system consists of antioxidant enzymes and non-enzyme antioxidants. A lot of antioxidants have been studied. Some are found in the body, others are found in nature and others are produced chemically. It is clear, therefore, that the study of antioxidants, which can prevent or suppress oxidative stress, is of crucial importance to the health of the skin and more generally to the human being.
In this thesis the importance of hydrophilic antioxidants, namely ascorbic acid, uric acid and glutathione, will be evaluated in muscle skin normal and inflammatory under different conditions each time. For each experiment a different animal model, other irradiation conditions, and different treatment will be used. Their change under the influence of ultraviolet radiation and the correlation between them will be examined too. At the same time, the effect of ultraviolet radiation on diabetic muscle skin will also be assessed.
The results indicate that exposure to ultraviolet radiation leads to a reduction in antioxidant molecules, thereby enhancing the production of free radicals. In the epidermis their concentration was higher than that of the dermis, irrespective of irradiation. Also the protective role of antioxidants, which can prevent or suppress oxidative stress, was reinforced. Finally, the sensitivity of diabetic skin to ultraviolet light relative to normal skin was perceived.
