Unit:
Κατεύθυνση Βιομηχανική ΧημείαLibrary of the School of Science
Author:
Kakoliri Athanasia
Supervisors info:
Λιούνη Μαρία, Αναπληρώτρια Καθηγήτρια, Τμήμα Χημείας, ΕΚΠΑ
Μαγιάτης Προκόπης, Αναπληρωτής Καθηγητής, Τμήμα Φαρμακευτικής, ΕΚΠΑ
Κοΐνης Σπύρος, Αναπληρωτής Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Original Title:
ΑΝΑΠΤΥΞΗ ΜΕΘΟΔΟΥ ΓΙΑ ΤΟΝ ΠΟΣΟΤΙΚΟ ΠΡΟΣΔΙΟΡΙΣΜΟ ΤΗΣ ΡΕΣΒΕΡΑΤΡΟΛΗΣ ΚΑΙ ΤΟΥ ΓΛΥΚΟΖΙΤΗ ΤΗΣ ΜΕ ΧΡΗΣΗ ΦΑΣΜΑΤΟΣΚΟΠΙΑΣ 2D-NMR ΚΑΙ ΕΦΑΡΜΟΓΗ ΣΕ ΜΟΝΟΠΟΙΚΙΛΙΑΚΟΥΣ ΟΙΝΟΥΣ ΤΗΣ ΖΑΚΥΝΘΟΥ
Translated title:
DEVELOPMENT OF METHOD FOR THE QUANTITATIVE DETERMINATION OF RESVERATROL AND PICEID USING 2D-NMR SPECTROSCOPY AND APPLICATION IN UNICELLULAR WINES OF ZAKYNTHOS
Summary:
The aim of this thesis is the quantitative analysis of resveratrol and its derivative piceid found in wine with a new method of determination using NMR Spectroscopy. In particular, the total concentration of trans-resveratrol (trans-resveratrol and trans-piceid) was determined in various unicellular wines produced in Zakynthos. Four local grape varieties were analyzed and compared based on variety, climate and altitude.
Trans-resveratrol is a stilbenoid compound of plant origin belonging to the category of phytoalexins and is characterized by increased anti-oxidative and anti-inflammatory action. It‘s mainly found in various parts of the vineyard plant from where it’s transferred to the wine and its production is increased under conditions of biotic and abiotic stress.
In order to determine the resveratrol, initially the phenolic fraction of the wine was isolated by means of a column packed with an ion-exchange resin. Next analysis was performed using NMR spectroscopy. First, were obtained one-dimensional NMR spectra (1H-NMR) but due to their complexity that makes it difficult to determine and quantitatively analyze our compound was used a two-dimensional NMR spectroscopy (1H-1H COSY) in order to determine its total amount without the presence of other overlapping signals. After the identification of peaks based on the resonance frequency, the phases were processed with the appropriate software.
The one-dimensional and two-dimensional NMR analysis techniques can give a very good picture of the total concentration of trans-resveratrol as well as information about the composition of the wine concerning phenolic substances which can serve as a «fingerprint» for the identification of each variety.
Main subject category:
Science
Keywords:
Resveratrol, Piceid, Unicellular wines of Zakynthos, NMR Spectroscopy, Quantitative analysis
Number of references:
134
