Dissertation committee:
Ε. Κιτράκη, Καθηγήτρια, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Χ. Ραχιώτης, Επίκουρος Καθηγητής,Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Ν. Κερεζούδης, Αναπληρωτής Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Α. Κακάμπουρα, Καθηγήτρια, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Γ. Ηλιάδης, Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Π. Πανόπουλος, Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Ι. Τζούτζας, Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Summary:
Early responses of human pulp to Prime&Bond / phosphoric acid, Clearfil SE Bond, Clearfil S3 Bond and Dycal were investigated ex vivo.
The three adhesives, Dycal or buffer (DPBS) were applied directly onto the pulp of human teeth slices that were placed in culture for 4 days. Cell viability was monitored by the MTT assay during the culture period. After 4 days, tissue integrity was examined by hematoxylin-eosin staining, Vimentin immunostaining and Western blotting, and TUNEL assay for apoptotic cells. Endoplasmic reticulum stress and tissue repair were assessed through the levels of ERdj5 and TGFβ2 determined by immunohistochemistry and Western blotting.
Profound reduction of cell viability and tissue integrity was observed in adhesives’-treated groups, while the impact of Dycal was less harmful. Extended apoptosis was caused by the Clearfil SE and Prime&Bond, while in Dycal group apoptosis was limited to the application area. All adhesives reduced Vimentin and TGFβ2 levels at the application site. Total Vimentin levels were reduced as well. Increased ERdj5 levels were detected in adjacent to the treatment areas of Prime&Bond- and Dycal-treated groups.
These results provide evidence that the early pulp responses to different capping materials may vary significantly and underline the need for further mechanistic studies in relevant ex vivo systems.
Keywords:
Pulp, Direct capping, Adhesive systems, Dycal, Apoptosis, Vimentin
