Combined study on the single nucleotide polymorphisms in the FSH and AMH receptor as genetic markers in assisted reproduction technology.

Postgraduate Thesis uoadl:2800066 334 Read counter

Unit:
ΠΜΣ Αναπαραγωγική-Αναγεννητική Ιατρική
Library of the School of Health Sciences
Deposit date:
2018-09-28
Year:
2017
Author:
Giannelou Polina
Supervisors info:
Δημήτριος Λουτράδης, Καθηγητής, Ιατρική, ΕΚΠΑ
Πέτρος Δρακάκης, Αναπληρωτής Καθηγητής, Ιατρική, ΕΚΠΑ
Κωνσταντίνος Καλλιανίδης, Αναπληρωτής Καθηγητής, Ιατρική, ΕΚΠΑ
Original Title:
Συνδυαστική μελέτη συσχετισμού των πολυμορφισμών στους υποδοχείς της FSH και της AMH σε πτωχές και καλές απαντήτριες στην IVF.
Languages:
Greek
Translated title:
Combined study on the single nucleotide polymorphisms in the FSH and AMH receptor as genetic markers in assisted reproduction technology.
Summary:
Aim
The purpose of the present study was to investigate whether the combination of two polymorphisms found on human FSH receptor gene – FSHR (Ser680Asn), and on human AMH receptor gene - AMHRII (−482 A>G), respectively are related to the assisted reproduction outcome.
Methods
In this study we assessed the distribution of the genotypes of FSHR Ser680Asn and of AMHRII −482 A>G gene polymorphisms in an IVF/ICSI group consisting of 126 women undergoing IVF/ICSI treatment for infertility and a control group consisting of 100 fertile women. Genotypes were determined using real-time PCR to discriminate for the different genotypes. We also performed a combinational study of the 9 possible combinations of the two polymorphisms and investigated their respective frequency in Greek women.
Results
Real-time PCR genotyping results revealed three different genotypes for each gene; women homozygous for the normal allele, women heterozygous for the polymorphism and women homozygous for the polymorphism. Statistical analysis showed that the frequency of the genotypes is similar in both control and IVF/ICSI groups. Further investigation of the frequency of the 9 possible combinations of these polymorphisms in the groups revealed no correlation between infertility and combination of the polymorphisms.
Conclusions
Our results show that in the Greek population the distribution of the FSHR Ser680Asn and of the AMHRII −482 A>G gene polymorphisms, is similar in fertile and infertile women and that there is no correlation of clinical importance between these polymorphisms and infertility.
Main subject category:
Health Sciences
Keywords:
FSH, AMH, FSHR, AMHRII, SNPs, Genetic markers, ART
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
116
Number of pages:
102
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