Η μελέτη της δυναμικής συμπεριφοράς των μιτοχονδρίων και των ρυθμιστών τους  στην ψωρίαση

Θεριανού Αναστασία

Unit:

Τομέας Κλινικοεργαστηριακός
Library of the School of Health Sciences

Deposit date:

2018-11-06

Year:

2018

Author:

Therianou Anastasia

Dissertation committee:

1. Θεοχάρης Θεοχαρίδης, Καθηγητής, Ιατρική Σχολή Πανεπιστημίου Tufts, Βοστώνη, ΗΠΑ.
2. Αλέξανδρος Κατούλης, Αναπληρωτής Καθηγητής , Ιατρική, ΕΚΠΑ
3. Ηλέκτρα Νικολαΐδου, Αναπληρώτρια Καθηγήτρια, Ιατρική, ΕΚΠΑ
4. Δημήτριος Ρηγόπουλος, Καθηγητής , Ιατρική, ΕΚΠΑ
5. Αλέξανδρος Στρατηγός, Καθηγητής, Ιατρική, ΕΚΠΑ
6. Ερυφίλη Χατζηαγγελάκη, Αναπληρώτρια Καθηγήτρια ,Ιατρική, ΕΚΠΑ
7. Αργυρώ Χατζηΐωάννου, Αναπληρώτρια Καθηγήτρια, Ιατρική, ΕΚΠΑ

Original Title:

Η μελέτη της δυναμικής συμπεριφοράς των μιτοχονδρίων και των ρυθμιστών τους στην ψωρίαση

Languages:

Greek

Translated title:

Mitochondria and their regulators in psoriasis .

Summary:

Psoriasis is characterized by keratinocyte proliferation and chronic inflammation, but the pathogenesis is still unclear. Dysregulated mitochondria could lead to reduced apoptosis and extracellular secretion of mitochondrial DNA (mt DNA), acting as “innate pathogen” triggering inflammation. Serum was obtained from healthy volunteers and psoriatic patients. Mitochondrial DNA was extracted from the serum and amplified with quantitative PCR (qPCR). Punch biopsies were obtained from lesional and non-lesional psoriatic skin (10 cm apart) and from healthy volunteers, were placed in RNA later and were stored at -80°C until RNA was extracted and cDNA was synthesized; gene expression of uncoupling protein 2 (UCP2), Dynamin-related protein 1 (Drp1) and calcineurin, involved in the regulation of mitochondria function, was detected with qPCR. Mitochondrial DNA was significantly increased (7s, p=0.0496 and Cytochrome B, CytB, p=0.0403) in the serum of psoriatic patients (n=63) as compared to controls (n=27). Gene expression was significantly reduced for UCP2 (p=0.0218), Drp1 (p=0.0001) and calcineurin (p=0.0001) in lesional psoriatic skin, as compared to non-lesional or control skin. Increased serum extracellular mtDNA in psoriatic patients and decreased expression of mitochondrial regulatory proteins in psoriatic skin suggest increased inflammation and reduced keratinocyte apoptosis, respectively. Inhibitors of mtDNA secretion and/or UCP2 stimulants may be potential treatment options.

Main subject category:

Health Sciences

Keywords:

Mitochondria, Inflammation, Calcineurin, Drp1, UCP2, Psoriasis

Index:

Number of index pages:

Contains images:

Yes

Number of references:

148

Number of pages:

103