A Study of the Effect of Probiotics, Prebiotics and Symbiotics on Intestinal Mucosa and Intestinal Mucosal Barrier during Short Bowel Syndrome in Rats

Doctoral Dissertation uoadl:2865039 465 Read counter

Unit:
Faculty of Medicine
Library of the School of Health Sciences
Deposit date:
2019-03-07
Year:
2019
Author:
Stoidis Christos
Dissertation committee:
Παύλος Πατάπης, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Γαβριήλ Καρατζάς, Ομότιμος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Εμμανουήλ Πικουλής, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ευάγγελος Μισιακός, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Νικόλαος Ζάβρας, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ανέστης Χαραλαμπόπουλος, Επίκουρος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Κωνσταντίνος Νάστος, Επίκουρος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Η μελέτη της επίδρασης των προβιοτικών, πρεβιοτικών, συμβιοτικών στον εντερικό βλεννογόνο και στον εντερικό βλεννογονικό φραγμό κατά το σύνδρομο του βραχέος εντέρου σε επίμυες
Languages:
Greek
Translated title:
A Study of the Effect of Probiotics, Prebiotics and Symbiotics on Intestinal Mucosa and Intestinal Mucosal Barrier during Short Bowel Syndrome in Rats
Summary:
Background and Purpose: Beyond the limited recent information about the effect of symbiotics (SYMs) on enterocyte level during short bowel syndrome (SBS), there is no available evidence on systematic extra-intestinal level, especially concerning the phenomena of apoptosis and lipopolysaccharide (LPS) endotoxinemia. With this novel sudy we tested the hypothesis that symbiotics are effective as preventive or therapeutic measure given pre- or postoperatively accordingly, after subtotal small bowel resection not only locoregionally but also systematically.
Materials and Methods: In this two- week study we used male Wistar rats that were divided into seven groups (n= 10/group): a control group (non operated/ fed regular diet), a sham group (bowel transection and re- anastomosis/ fed regular diet), four experimental groups (75% small bowel resection/ ± symbiotic intake pre- or post OP) and a final group of non- operated animals fed with SYMs enriched diet. Intestinal structural changes (villus height, lymphocyte mucosal infiltration), apoptosis (intestinal, liver, splenic, pulmonary and cardiac cells), LPS serum levels and bacterial translocation (BT) of gram negative intestinal bacteria to mesenteric lymph nodes, liver, spleen, and peripheral blood were determined.
Results: Treatment with SYMs in bowel resected subjects resulted in an increase in villus height (905.236±54.892 vs 787.154±17.523 μm, p<0.05) and in a decrease in BT (80% vs 100%, p>0.05), apoptotic index (110.188±2.824 vs 99.302±3.284, p<0.05), lymphocyte mucosal infiltration (30% vs 50%, p>0.05) and LPS serum levels (1.67±0.322 vs 3.831±1.544 eu/ml, p<0.05) compared with untreated animals.
Conclusions: In a rat model SYMs seem to enhance the intestinal barrier and protect the cellular structure from the local and systematic effects of SBS.
Main subject category:
Health Sciences
Keywords:
Symbiotics; Intestinal mucosa; Intestinal barrier; Short bowel syndrome; Animal model
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
230
Number of pages:
165
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