Unit:
Κατεύθυνση Αναλυτική ΧημείαLibrary of the School of Science
Author:
Thanasoula Ioanna
Supervisors info:
Μπακέας Ευάγγελος, Αναπληρωτής Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Original Title:
Ανάπτυξη και επικύρωση μεθόδου προσδιορισμού βιογενών οργανικών ρύπων
Translated title:
Development and validation of determination of biogenic organic compounds method
Summary:
In this MSc Thesis, a GC-MS method was developed and validated for the determination of secondary organic compounds and their distribution between PM10 and PM2.5 particles.
PM10 and PM2.5 sampling was conducted using glass fiber filters with a large volume sampler. The filter pretreatment for the isolation of the secondary organic compounds involves extraction with dichloromethane / methanol via ultrasonic waves, concentration on a rotary evaporator and concentration in a nitrogen stream finally. Due to the polar groups in the oxidized forms, the need for derivatization is imperative. For this reason, two different multistep derivatization methods were tested and the differentiation between them was in the second step. First method’s, second step was adding TMSD and MEOH while second method’s second step was adding BF3 / MeOH. The method with the best response and better quality of chromatograms was the first method. Additionally in finding optimal derivatization conditions, tests were performed on specific parameters. Finally, a gas chromatographic determination follows.
The validation of the method was performed through the examination of quality characteristics such as specificity, linearity, presicion, recoveries and detection and quantification limits. The method showed satisfactory results (recoveries from 41 to 118%). The detector response was linear in the range of 0.5 to 10 μg / mL.
Eventually, the method has been successfully applied to real samples from areas of Cyprus.
Main subject category:
Science
Keywords:
biogenic, SOA, optimization, validation, gas-chromatography, mass spectometry
