Unit:
Τομέας ΧειρουργικήςLibrary of the School of Health Sciences
Author:
Prodromidou Anastasia
Dissertation committee:
Γεώργιος Βάος, Καθηγητής, Ιατρική, ΕΚΠΑ
Δέσποινα Περρέα, Καθηγήτρια, Ιατρική, ΕΚΠΑ
Αικατερίνη Παυλάκη, Καθηγήτρια, Ιατρική, ΕΚΠΑ
Νικόλαος Νικητέας, Καθηγητής, Ιατρική, ΕΚΠΑ
Δημήτριος Δημητρούλης, Αναπληρωτής Καθηγητής, Ιατρική, ΕΚΠΑ
Παπαλάμπρος Αλέξανδρος-Λουκάς, Επίκουρος Καθηγητής, Ιατρική, ΕΚΠΑ
Δημήτριος Σχίζας, Επίκουρος Καθηγητής, Ιατρική, ΕΚΠΑ
Original Title:
Η πιθανή ευεργετική αυξητικών παραγόντων στην επούλωση πειραματικά δημιουργηθείσας και εκταμθείσας ενδομητριωσικής εστίας στο παχύ έντερο επίμυων
Translated title:
Healing After Resection of Experimentally Induced Colonic Endometriosis and Application of Growth Factor Enriched Agents: An Experimental Rat Model
Summary:
Study objective: To examine the potential beneficial effect of platelet rich plasma (PRP) and fibrin sealant (TISSEEL; Baxter Healthcare Corporation, Deerfield, IL) on bowel wound healing after shaving of an experimentally induced endometriotic lesion.
Design: Single-blind randomized experimental study (Canadian Task Force classification I).
Setting: Certified animal research facility.
Animals: Thirty female Sprague-Dawley rats.
Interventions: Experimental colonic endometriosis was induced by transplanting endometrial tissue to all animals (first surgery). Thirty rats were then randomized to 1 of 3 groups according to treatment: PRP (group I, n = 10), fibrin sealant (group II, n = 10), or no agent (group III, n = 10) was applied after shaving of the endometriotic nodule (second surgery).
Measurements and Main Results: Colonic endometriosis was successfully induced in all subjects. Four days following the second surgery, the animals were euthanized, and microscopic evaluation was performed. The pathologist was blinded to the treatment method. Histopathologic analysis revealed that compared with the control group, collagen disposition was found in significantly higher expression in both the PRP and fibrin sealant groups (p = .011 and p = .011, respectively). Distortion of the integrity of the colon layers was statistically more pronounced in the control group compared with the fibrin sealant group (p = .033) while greater new blood vessel formation was observed in the fibrin sealant group compared with control (p = .023). No histologic evidence of residual or recurrent disease was detected.
Conclusion: Both PRP and fibrin sealant appear to be safe and associated with improved tissue healing during shaving for the excision of colonic endometriosis, attributed to the enhanced collagen disposition, neovascularization, and protection of the integrity of colon layers. Clinical trials are warranted to confirm the feasibility of PRP and fibrin sealant in the clinical setting.
Main subject category:
Health Sciences
Keywords:
Endometriosis, Colorectal endometriosis, PRP, Fibrin sealants, Healing, Shaving
Number of references:
132
