Unit:
Department of DentistryΒιβλιοθήκη Οδοντιατρικής
Dissertation committee:
Πανόπουλος Παναγιώτης, [επιβλέπων] Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ.
Cerutti Antonio, Καθηγητης University of Brescia Italy.
Ζηνέλης Σπυρίδων, Αναπληρωτής Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ.
Κερεζούδης Νικόλαος, Αναπληρωτής Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ.
Φαρμάκης Ελευθέριος, Επίκουρος Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ.
Αγραφιώτη Αναστασία, Επίκουρη Καθηγητρια, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ.
Τζανετάκης Γεώργιος, Επίκουρος Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ.
Original Title:
Self-adjusting file (SAF) compared to newly designed rotary and reciprocating files: composition and microstructure, microbial biofilm removal capability and shaping effectiveness.
Translated title:
Self-adjusting file (SAF) compared to newly designed rotary and reciprocating files: composition and microstructure, microbial biofilm removal capability and shaping effectiveness.
Summary:
The aim of this study is to compare the Self adjusting file (SAF) to Wave One (WO) , BT-Race (BTR) and manual instrumentation (MAN) in their basic composition through SEM-EDX spetroscopy, the ability in disinfecting ex vivo long oval canals after E.Faecalis contamination though cultures and the cleanliness of the root canal space for debris and smear layer through SEM and Optical microscopy after chemomechanical preparation.
Files were analysed through SEM-EDX spectroscopy for the basic composition. All files analysed contained Ni and Ti. The WO contained 52,14%wt. of Ni and 46,79%wt. of Ti, the SAF 51,99%wt. of Ni and 47,12 %wt. Ti and the BTR 51,49%wt. of Ni and 47,31%wt. of Ti. No differences were observed between the groups.
For the microbiological experiment 100 long oval teeth were collected and after initial preparation and contamination with E.Faecalis were divided into four groups randomly (n=25). Before chemomechanical preparation the first microbiological sampling was performed (S1). Afterwards instrumentation was performed according to the protocol of each instrument and the second sampling performed afterwards (S2). From the results in S1 between the groups no difference was observed. the reduction from S1to S2 in all four groups was significant while in the comparison in S2 between the groups MAN reduced significantly less the CFU counts in comparison to the other tree groups (p<0,001) ant the SAF reduced significantly less the bacterial counts compared to BTR (P<0,01).
For the cleaningness experiment five teeth from each group were selected and separated in their longitudinal axis and observed under the optical microscope (200x) for debris and under the SEM (200x and 1000x) for debris and smear layer. Two independent examiners evaluated the findings attributing scores from 1-4. From the average scores resulted that MAN and SAF were significantly less effective to BTR and WO in cleaning the root canal in the debris and smear layer study. The cleaner location was the coronal with significant difference to the apical. Between the two observation techniques in the debris study no significance was found.
Concluding all three Ni-Ti instruments performed better compared to manual instrumentation.
The self adjusting file did non perform as well as the reciprocating and the rotating system.
None of the instruments tested achieved perfect score in cleaning the root canal and disinfecting long oval canals.
Main subject category:
Health Sciences
Keywords:
Self adjusting file, Wave One, BT Race, Chemomechanical Preparation, Effectiveness of Rotary Instruments, E faecalis.
Number of references:
428
