Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Author:
Polyzos Panagiotis
Dissertation committee:
Αγαπητός Εμμανουήλ, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ, Επιβλέπων
Τσελένη-Μπαλαφούτα Σοφία, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Παυλάκη Αικατερίνη, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Ευαγγέλου Κωνσταντίνος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Κουλουκουσα-Γιαννιού Μυρσίνη, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Μαρίνος Ευάγγελος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Χαβάκη Σοφία, Επίκουρη Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Συγκριτική μελέτη της έκφρασης της κερατίνης 8 και της γλυκοζυλιωμένης ομάδας H, στο παραγωγικό, στο εκκριτικό ενδομήτριο και στο φθαρτό
Translated title:
Comparative study of the expression of keratin 8 and its glycosylated group H, in the proliferative, in the secretory endometrium, and in the decidua
Summary:
In the present study , thirty cases of proliferative endometrium, thirty cases of early secretory endometrium, thirty cases of middle secretory endometrium, thirty cases of late secretory endometrium and thirty cases of decidual tissues were investigated immunohistochemically in order to detect in these tissues the expression of the cytokeratin 8 and its glucosylated epitope H which bears the sugar O-N- acetylglucosamine in a specific conformation and /or environment. The study provided the following results:
1) The cytokeratin 8 is expressed in the epithelial cells (surface and glandular epithelium) of the endometrium during the whole legth of the menstrual cycle.
2) The cytokeratin 8 is expressed in the epithelial cells (surface and glandular epithelium ) of the decidua.
3) The cytokeratin 8 is not expressed in the mesenchymal components (stromal and blood vessel wall cells) of the endometrium and decidua.
4) The cytokeratin 8 is overexpressed in the glandular epithelium of the secretory endometrium and deciduas compared to the glandular epithelium of the proliferative endometrium.
5) The expressions of the of the cytokeratin 8 and its glucosylated epitope H are parallel and equal in the endometrial glands during the whole length of the menstrual cycle and in the decidual glands meaning that the rate of the cytokeratin 8 glycosylation remains constant through the duration of the menstrual cycle and in the decidua.
6) The decidual cells presented in all cases high expression of the epitope H in their cytoplasm.
7) The predecidual cells of the late secretory endometrium in all cases presented significant expression of the epitope H in their cytoplasm ranging from moderate to high expression.
8) The non predecidual stromal cells of the functional endometrial layer in all cases presented insignificant expression of the epitope H in their cytoplasm ranging from negative to low expression.
9) The stromal cells of the basal layer of the endometrium and decidua did not present the epitope H in the cases.
10) The endometrial stromal granular cells did not present the epitope H in all cases.
11) The blood vessel wall cells (endothelial and smooth muscle) of the endometrium through the whole duration of the menstrual cycle and of the decidua presented high expression of the epitope H in their cytoplasm.
Finally, it is concluded, that the endometrial stromal cells of the functional layer of the endometrium without producing the cytokeratin 8, produce a protein or proteins which carry the epitope H of the cytokeratin 8 which bears the sugar residue O-N- acetylglucosamine.
Main subject category:
Health Sciences
Keywords:
Keratin 8, Glycosylated group H, O-GlcNAcH, Endometrium, Decidua, O-GlcNAcylation
