Unit:
Κατεύθυνση Φαρμακευτική Ανάλυση - Έλεγχος ποιότηταςLibrary of the School of Science
Author:
Papaioannou Angeliki
Supervisors info:
Κωνσταντίνος Γιαγκίνης, Αναπληρωτής Καθηγητής, Τμήμα Επιστήμης Τροφίμων & Διατροφής, Πανεπιστήμιο Αιγαίου
Original Title:
Μελέτη χρωματογραφικών δεικτών λιποφιλίας φλαβονοειδών αναλόγων με την χρήση υγρής χρωματογραφίας αντιστρόφου φάσης (στήλη LC-18-DB) και η σχέση τους με την εντερική διαπερατότητά τους
Translated title:
Μελέτη χρωματογραφικών δεικτών λιποφιλίας φλαβονοειδών αναλόγων με την χρήση υγρής χρωματογραφίας αντιστρόφου φάσης (στήλη LC-18-DB) και η σχέση τους με την εντερική διαπερατότητά τους
Summary:
Flavonoids are a group of natural products, of plant origin, belonging to the chemical class
of polyphenols. They present a variety of chemical structures and characteristics, as well
as important pharmacological properties. Their beneficial effects include antioxidant,
antimicrobial, anti-inflammatory and anti-atherosclerotic. The most critical parameter for
the manifestation of their biological activity is thought to be their absorption through the
small intestinal mucosa. Lipophilia of a substance significantly determines its absorption
through the intestinal epithelium.
In the present study, the chromatographic logkw indices of 29 flavonoids were determined
by the use of reversed phase high performance liquid chromatography (RP-HPLC).
SUPELCOSIL LC-18-DB was used as static phase. For the preparation of mobile phase,
octanol-saturated MOPS buffer with pH 7.4 was used aqueous component and methanol
as organic modifier. A large number of physicochemical properties of flavonoids were
determined using software programs. The intestinal permeability data of the flavonoids
studied were collected from the literature.
A similar elution mechanism was found for all flavonoids with minor deviations. A
moderate linear correlation was observed between logkw chromatographic indices and
intestinal permeability coefficient for 21 flavonoids for which bibliographic data were
available. The introduction of the term logD7,4 significantly improved the correlation
between the logkw indices and the intestinal permeability coefficient. The resulting
equation could be used to estimate the intestinal permeability of flavonoids for which no
experimental values are available in the literature.
Main subject category:
Science
Keywords:
Flavonoids, Reverse Phase High Performance Liquid Chromatography (RP-HPLC), Lipophilicity, Intestinal Absorption, Caco-2 cells, LC-18-DB
