Unit:
Library of the School of Health Sciences
Dissertation committee:
Γρηγόριος Κουράκλης, Ομότιμος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ανδρέας Πολυδώρου, Καθηγητής, , Ιατρική Σχολή, ΕΚΠΑ
Ευστάθιος Μποβιάτσης, Καθηγητής, , Ιατρική Σχολή, ΕΚΠΑ
Κωνσταντίνος Κόνζτογλου Καθηγητής, , Ιατρική Σχολή, ΕΚΠΑ
Ευστάθιος Αντωνίου, Αναπληρωτής Καθηγητής, , Ιατρική Σχολή, ΕΚΠΑ
Δημήτριος Δημητρούλης, Αναπληρωτής Καθηγητής, , Ιατρική Σχολή, ΕΚΠΑ
Χρήστος Σαλάκος Αναπληρωτής, Καθηγητής, , Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Δημιουργία ικριώματος από ανθρώπινο ομφάλιο λώρο για την αποκατάσταση δυσλειτουργιών του περιφερικού νευρικού συστήματος.
Translated title:
Scaffold from human umbilical cord for injured peripheral nerve repair
Summary:
One of the biggest challenges in surgery is the restoration of the peripheral nerves. Despite the great advances in microsurgery in cases where there is a gap between the nerve stumps, complete recovery of nerve function has not been achieved. In such lesions, the use of nerve conduit is necessary for bridging the gap between the nerve stumps. A wide variety of biomaterials are being used to promote functional recovery of injured nerves, such as autologous nerve graft, synthetic materials that are non-biodegradable, or biodegradable, natural materi-als such as collagen, chitosan, and keratin. Furthermore, various tissues of non-neural origin such as vessels, muscles, and intestine submucosa, have been used as nerve conduits.
The aim of this study is to accomplish a successful decellularization of the human umbilical artery (hUA) and then its use as a nervous guide.
Initially, the hUAs were decellularized, with the use of hypertonic solutions and detergents. Subsequently, evaluation of the decellularization performed, with the use of histological stains, DNA quantification and cytotoxicity analysis. Fur-thermore, a segmental peripheral nerve injury created in 24 Sprague–Dawley rats. The animals were organized into three experimental groups with different forms of repair: autologous nerve graft (n = 12), decellularized hUA (n = 12), de-cellularized hUA in combination with PRP (n = 12).
For functional recovery evaluation, sciatic faction index and gastrocnemius muscle weight ratio estimated. For the nerve fibre regeneration evaluation, nerve morphometry and immunohistochemistry analysis performed.
According to the results of this study, the hUA can support the proliferation of regenerated nerve fibers in a 10 mm nerve gap. Additionally, the combination of hUA and PRP, have the same results using an autologous nerve graft.
Main subject category:
Health Sciences
Keywords:
Scaffold, Nerve conduit, Umbilical cord, Decellularization, PRP.
Number of references:
217
