Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Dissertation committee:
Λιακάκος Θεόδωρος, Καθηγητής, Ιατρική σχολή, ΕΚΠΑ
Μισιακός Ευάγγελος, Καθηγητής, Ιατρική σχολή, ΕΚΠΑ
Χαραλαμπόπουλος Αλέξανδρος, Αν. Καθηγητής, Ιατρική σχολή, ΕΚΠΑ
Νικητέας Νικόλαος, Καθηγητής, Ιατρική σχολή, ΕΚΠΑ
Πατάπης Παύλος, Αν. Καθηγητής, Ιατρική σχολή, ΕΚΠΑ
Σχίζας Δημήτριος, Επικ. Καθηγητής, Ιατρική σχολή, ΕΚΠΑ
Παπαλάμπρος Αλέξανδρος, Επικ. Καθηγητής, Ιατρική σχολή, ΕΚΠΑ
Original Title:
Ιστικός και ποσοτικός προσδιορισμός των TLRs στο έντερο και στους νεφρούς σε σηπτικά μοντέλα μυών: πειραματική μελέτη
Translated title:
Increased expression of Toll-like receptors 2, 3, 4 and 7 mRNA in the kidney and intestine of a septic mouse model
Summary:
Background: Toll-like receptors (TLRs) are key regulators of innate and adaptive immunity and are highly expressed during sepsis. Thus, studying the expression of TLRs in an animal septic model might indicate their possible association with acute kidney injury (AKI) in sepsis.
Methods: Seventy-two male C57BL/6J mice were used for this study. Randomly, these animals were divided into 6 groups (N=12/group): 3 control and 3 septic groups depending on the euthanasia time (24h, 48h, 72h). Septic groups underwent cecal ligation and puncture (CLP) to induce peritonitis, while control groups had a sham operation. Hematological tests were performed in serum for immune biomarkers; immunofluorescence (IF), morphometry and qRT-PCR analysis was used on both kidney and intestine tissues to evaluate the expression of TLRs 2, 3, 4 and 7 in a septic process.
Results: At the end of each experimental period, we found that TLRs 2, 3, 4 and 7 were expressed in both tissues but there were differences between those at various time points. Also, we found that mRNA levels were significantly higher in qRT-PCR evaluation in septic groups than control groups in both kidney and intestinal tissues; showing a steady increase in the septic groups as time to euthanasia was prolonged (p<0.05).
Conclusion: Overall, our study provides a suggestion that TLRs 2, 3, 4 and 7 are highly increased in the kidneys of septic mice and especially, that these TLRs are sensitive and specific markers for sepsis. Finally, our study supports the diagnostic importance of TLRs in AKI and provides new data about them in septic mice models and in multi organ dysfunction syndrome (MODS) in general.
Main subject category:
Health Sciences
Keywords:
Sepsis, Kidney, TLR, MODS
Number of references:
407
