Unit:
Κατεύθυνση Απομόνωση, Ανάπτυξη, Παραγωγή και Έλεγχος Βιοδραστικών Φυσικών ΠροϊόντωνLibrary of the School of Science
Author:
Kagkiouzi-Pontmpielski Ira-Labrini
Supervisors info:
Προκόπιος Μαγιάτης, Αναπληρωτής Καθηγητής, τμήμα Φαρμακευτικής, ΕΚΠΑ
Ιωάννα Χήνου, Καθηγήτρια, τμήμα Φαρμακευτικής, ΕΚΠΑ
Ελένη Σκαλτσά, Καθηγήτρια, τμήμα Φαρμακευτικής, ΕΚΠΑ
Original Title:
MEΛΕΤΗ ΒΟΤΑΝΙΚΩΝ ΕΓΧΥΜΑΤΩΝ ΑΠΟ ΕΙΔΗ SIDERITIS, CAMELLIA, OLEA KAI MENTHA ΚΑΙ ΠΟΣΟΤΙΚΟΣ ΠΡΟΣΔΙΟΡΙΣΜΟΣ ΤΩΝ ΚΥΡΙΩΝ ΒΙΟΔΡΑΣΤΙΚΩΝ ΣΥΣΤΑΤΙΚΩΝ ΣΤΑ ΕΓΧΥΜΑΤΑ ΜΕ ΧΡΗΣΗ ΠΟΣΟΤΙΚΗΣ ΦΑΣΜΑΤΟΣΚΟΠΙΑΣ NMR
Translated title:
Study of botanical infusions from Sideritis, Camellia, Olea and Mentha species and quantitation of the main bioactive compounds in infusions by qNMR
Summary:
In the framework of the present thesis, a qualitative and quantitative analysis of the main active compounds of herbal teas known from the ancient time for their traditional use, was carried out. This was fulfilled in order to investigate the quantity of each compound that can be transferred from the herb to the infusion. For this purpose, an 1H-NMR (Nuclear magnetic resonance) investigation was performed on the herbal teas. The herbs included in the current study were 6 species of the genus Sideritis spp., pulverised leaves of 26 varieties of Camellia sinensis L., leaves of two varieties of Olea europea L., as well as pulverised leaves of Mentha x piperita. Their infusions were prepared according to a specific protocol, followed by measurement of the peaks of their main ingredients in the 1H-NMR spectra. In some species of the genus Sideritis further preparation of the samples was carried out, based on the proportions described in the monograph of the Committee on Herbal Medicinal Products (European Medicines Agency).
Specifically, from the genus Sideritis the dominant constituents were identified in: Sideritis raeseri Boiss. & Heldr. (from mount Parnassos), Sideritis clandestina subsp. clandestina (from mount Taygetos and Parnon), Sideritis syriaca L. (from Crete Island), Sideritis scardica Griseb. (from mount Olympos), Sideritis euboea Heldr. (from Euboea island) and Sideritis sipylea Boiss. (from Lesvos island). In order to confirm the identity of the main ingredients, some of them had to be isolated and the rest were identified with chromatographic comparison with substances that had already been isolated in the laboratory. The NMR data of the isolated substances were compared with those mentioned in the literature.
Subsequently, we prepared infusions separatelly from the leaves, the stems and the flowers of the species Sideritis clandestina subsp. clandestina based on the proportions described by the HMPC (EMA). Τhis study was performed to determine which part of the plant was the richest source of the main substances found in the infusion. Αfter the study of every separate herbal tea, the main purpose was to identify the same active components in a mixture consisted of the specific herbs in specific proportions. Thus, these bioactive substances were simultaneously identified and quantitated with a single 1H-NMR spectrum recorded for a mixture of olive leaf-mint-sideritis infusion and olive leaf-mint-green tea infusion.. This technique gave us the opportunity to measure simultaneously and in only a few seconds the main flavonoids and iridoids of Sideritis, caffeine and catechins of Camellia sinensis, rosmarinic acid of Mentha and oleuropein of olive leaves.
Main subject category:
Science
Other subject categories:
Health Sciences
Keywords:
botanical infusions, Sideritis, Camellia sinensis, quantitation, qNMR
