Unit:
Κατεύθυνση Επιστήμη Πολυμερών και Εφαρμογές της στη ΒιομηχανίαLibrary of the School of Science
Supervisors info:
Ιατρού Ερμόλαος, Καθηγητής, Τμήμα Χημείας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Μαρίνος Πιτσικάλης, Καθηγητής, Τμήμα Χημείας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Γεώργιος Σακελλαρίου, Αναπληρωτής Καθηγητής, Τμήμα Χημείας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Original Title:
Σύνθεση και χαρακτηρισμός Νε-FMOC-προστατευμένου Ν-Καρβόξυ Ανυδρίτη L-Λυσίνης και εμβολιασμένου συμπολυμερούς πολυ(L-λυσίνης) και πολυ(L-ιστιδίνης)
Translated title:
Synthesis and characterization of Nε-FMOC-protected N-Carboxy Anhydride L-Lysine and graft copolymer of poly (L-lysine) and poly (L-histidine)
Summary:
During the present research work, the synthesis and characterization of protected N-carboxy anhydrides (monomers) as well as pH-responsive hybrid copolymers of the type PLL78-b- (PLL10-g-PHis15) were achieved. The polymer was synthesized by ring-opening polymerization of the corresponding Ncarboxy anhydrides with the use of hexylamine as initiator. In order to achieve the synthesis of well-defined polymer it was necessary to use N-carboxy anhydrides with different protective groups so that there was the possibility of selective deprotection in different conditions. In order to achieve the synthesis of well-defined polymers, ensure the purity of the solvents and therefore the purity of the whole system so to avoid non controlled polymerization and termination reactions, high vacuum techniques were applied. The characterization of the polymer in order to confirm the successful synthesis as well as to define its molecular characteristics was carried out by size exclusion chromatography (SEC), proton nuclear magnetic resonance (1H-NMR) and infrared spectroscopy (FT-IR). Finally, dynamic light scattering (DLS), zpotential technique and the technique of circular dichroism (CD) were used, in order to investigate the ability of the polypeptide to self-assemble in different environmental conditions. The ultimate goal of this research work was to study the FMOC protective group, which can be combined with the BOC protective group, providing us the possibility to synthesize polymers with different architectures.
Main subject category:
Science
Keywords:
polypeptides, ring-opening polymerization, N-carboxy anhydrides, protective groups, stimuli-responsive polymers
