Unit:
Κατεύθυνση Ιατρική Φυσική-ΑκτινοφυσικήLibrary of the School of Health Sciences
Supervisors info:
Κουρκουμέλης Νικόλαος, Αναπληρωτής Καθηγητής, Τμήμα Ιατρικής, Πανεπιστήμιο Ιωαννίνων (επιβλέπων)
Εμφιετζόγλου Δημήτρης, Καθηγητής, Τμήμα Ιατρικής, Πανεπιστήμιο Ιωαννίνων (Μέλος εξεταστικής επιτροπής)
Χατζηκακού Σωτήρης, Καθηγητής, Τμήμας Χημείας, Πανεπιστήμιο Ιωαννίνων (Μέλος εξεταστικής επιτροπής)
Original Title:
Μελέτη της επίδρασης της χημικής σταθεροποίησης και ψύξης στην ποιότητα του οστικού ιστού με φασματοσκοπία Raman
Translated title:
Study of the effect of chemical fixation and freezing in bone quality by Raman spectroscopy
Summary:
Raman spectroscopy is an optical technique, suitable for the chemical characterization of the composition of heterogeneous samples, such as bone tissues. Through the identification of molecule – specific vibrational modes of the sample’s chemical components, Raman spectroscopy allows the simultaneous exploration of mineral and organic phase of bone composition. Raman spectroscopy provides the ability of a non – invasive measurement of biological samples in vivo or ex vivo. Fresh hydrated tissues can be also examined along with tissues that have been fixed with fixatives or have been immersed in embedding media. Thus, by studying of physicochemical parameters, subtle changes can be detected in molecular structure, providing useful information for the bone quality.
Typically, storage and preservation are required for all biological samples until the spectroscopic or other analysis. The need to study the effects of these procedures on bone structure and quality has led to the development of different protocols for storage. Here, chicken bones were collected and fixed by the use of fixatives such as formaldehyde, 10 % neutral buffered formalin, saline solution and ethanol. Furthermore, additional samples were (snap-) freezed or were subjected to successive freeze-thaw cycles for different intervals.
By the spectroscopic analysis and the investigation of bone quality parameters we conclude that in most cases there are changes in bone structure and spectral artifacts associated with the fixation. Fixation protocols found to have a direct impact on the structure of collagen proteins. Conversely, freeze- thawing of bone tissues was found as a reliable method for preservation.
Main subject category:
Science
Keywords:
Raman spectroscopy, chicken bones, fixation, cryopreservation, bone quality, apatite.
Number of references:
143
