Unit:
Κατεύθυνση Εφαρμογές της Βιολογίας στην ΙατρικήLibrary of the School of Science
Supervisors info:
Ισιδώρα Παπασιδέρη, Καθηγήτρια, Τμήμα Βιολογίας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Αναστάσιος Κριεμπάρδης, Αναπληρωτής Καθηγητής , Τμήμα Βιοϊατρικών Επιστημών, Τομέας Ιατρικών Εργαστηρίων, Πανεπιστήμιο Δυτικής Αττικής
Μαριάννα Χ. Αντωνέλου, Επίκουρη Καθηγήτρια, Τμήμα Βιολογίας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών,
Original Title:
Επιδράσεις της Ιονίζουσας ακτινοβολίας στα ώριμα ερυθροκύτταρα
Translated title:
Επιδράσεις της Ιονίζουσας ακτινοβολίας στα ώριμα ερυθροκύτταρα
Summary:
Ionizing radiation is widely used in the medical Radiology for diagnostic purposes. The harmful effects of the radiological examinations are yet to be discussed in the research community. This current study investigates the effects on hemolytic parameters of red blood cells in vitro, before and after the exposure of low doses of X-ray radiation, in blood taken from human healthy volunteers.
Blood samples were collected from 30 healthy volunteers, in EDTA tubes (2,5ml). The tubes were exposured to doses of 32,52μGy (2*0,03mGy) and 187,84μGy (2*0,2 mGy) used for medical diagnostic X-rays, whereas control tubes were not exposed. The osmotic fragility test and the measurement of free hemoglobin in plasma (Harboe method), are tested before the irradiation and two hours after blood irradiation. In these particular doses, there has been an augmentation in the hemolysis and in free hemoglobin of plasma that might be induced by ROS and the senescence of irradiated red blood cells. Therefore, a Pearson’s correlation has been conducted between the two hemolytic parameters depending on the x-ray doses, gender and age. It was observed that low doses of medical x-rays may have higher effect on the age group of 20-40 than the group of 40-60, therefore it needs further investigation. Low doses of X-rays seem not to have an effect in the mean corpuscular fragility (MCF) index.
In conclusion, low doses X-rays do not affect the MCF index, in comparison with free plasma hemoglobin. In order to have more accurate results, it is important further studies to be conducted with a larger sample, while different techniques should be performed in order to study the morphology of erythrocytes, the composition of erythrocyte membrane and erythrocytes’ senescence markers. Last but not least, these data are correlated with age.
Main subject category:
Science
Keywords:
Ιονίζουσα Ακτινοβολία, Ακτίνες Χ, ερυθροκύτταρα, in vitro, ελεύθερη αιμοσφαιρίνη, MCF
Number of references:
112
