Faculty of Medicine
Library of the School of Health Sciences

2020-12-23

2020

Zografos Konstantinos

Μαρία Kυριακοπούλου Λυμπέρη, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ, Επιβλέπουσα
Κωνσταντίνος Τούτουζας, Καθηγητής , Ιατρική Σχολή, ΕΚΠΑ
Απόστολος Παπαλόης, Επισκέπτης Καθηγητής, Ιατρική Σχολή, Πανεπιστήμιο Harvard
Ευάγγελος Mενενάκος, Καθηγητής , Ιατρική Σχολή, ΕΚΠΑ
Δημήτριος Θεοδώρου, Καθηγητής , Ιατρική Σχολή, ΕΚΠΑ
Νικόλαος Μιχαλόπουλος, Επίκουρος Καθηγητής , Ιατρική Σχολή, ΕΚΠΑ
Δημήτριος Σχίζας, Επίκουρος Καθηγητής , Ιατρική Σχολή, ΕΚΠΑ

Πειραματική απομόνωση και μεταμόσχευση μεσεγχυματικών κυττάρων (Adipose tissue-derived stem cells) σε πρότυπο τοξικής ηπατικής βλάβης σε αρουραίους. Ιστοπαθολογική, μοριακή και βιοχημική μελέτη για την σύγκριση πρωτοκόλλων μεταμόσχευσης

Greek

Experimental isolation and transplantation of Adipose tissue-derived stem cells. Model of hepatic injury. Histopathological, Molecular and biochemical study for comparison of transplantation protocols.

Objective: Acetaminophen overdose has been associated with severe hepatic necrosis leading to acute liver failure. Preclinical data suggest that the transplantation of mesenchymal stem cells, a population of multi-potent cells that exhibit great capacities for tissue repair, can improve hepatic injury.
Materials and Methods: Acetaminophen was used to induce liver injury in 32 Wistar rats. Adipose tissue mesenchymal stem cells were transplanted via the portal vein or intrahepatic route directly onto the liver parenchyma. Hepatic biopsies were obtained, and histopathological / biochemical evaluation was performed in order to evaluate acetaminophen-induced injury following stem cell transplantation. Additionally, immunohistochemical evaluation of chromogranin expression was conducted in hepatic stellate cells and lobular cells. Immunohistochemistry for hepatic growth factor (HGF) was used to study the effect of stem cells on liver regeneration.
Results: According to our results, there was a statistically significant increase of the necrosis score on the 4th day, in Group 4 (SC-HEP), where mesenchymal stem cells (MSCs) where administered directly on the hepatic parenchyma; necrosis subsequently gradually decreased, up until the 14th sday. Furthermore, HGF expression levels exhibited gradual increase from the 4th to the 14th day, in Group 3 wistar rats, where MSCs were administered through the Portal Vein.
Conclusion: The study presents the effect of adipose tissue stem cells on liver regeneration, providing valuable insight into the effect of drug-induced hepatotoxicity and the response of the liver tissue. Further studies on a preclinical/clinical setting are deemed necessary for the confirmation of our results on a larger scale.

Health Sciences

Adipose tissue stem cells, Acetaminophen, Acute liver injury, Hepatic growth factor, Liver regeneratio

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https://pergamos.lib.uoa.gr/uoa/dl/object/2931566