Κατεύθυνση Φαρμακολογία
Library of the School of Science

2020-12-29

2020

Christoforidis Dimokritos

Ανδρέας Παπαπετρόπουλος, Καθηγητής, Τμήμα Φαρμακευτικής, ΕΚΠΑ

Συγκριτική μελέτη της σηματοδότησης του H2S που παράγεται από την γ-λυάση της κυσταθειονίνης (CSE) και την 3-μερκαπτοπυροσταφυλική σουλφοτρανσφεράση (3-MST)

Greek

Comparison of signaling pathways triggered by H2S produced by cystathionine γ-lyase vs 3-mercaptopyruvate sulfurtransferase

Hydrogen sulfide (H2S) is the third identified gasotransmitter, after nitric oxide (NO) and carbon monoxide (CO). In mammalian cells, H2S is endogenously produced by the enzymes cystathionine γ-lyase (CSE), cystathionine β-synthase (CBS) and 3-mercaptopyruvate sulfurtransferase (3-MST), as well as by non-enzymatic pathways. H2S producing enzymes are the main intracellular source of H2S and they are regulated on many levels and by a number of factors. H2S plays an important role as a signaling molecule and exerts various biologic effects. Numerous studies focus on the molecular mechanisms and signaling pathways in which H2S and H2S producing enzymes are involved in physiological and pathophysiological conditions, in order to develop specific pharmacological tools with therapeutic potential.
In the present study, we studied the differences in signaling between the H2S-producing enzymes CSE and 3-MST in mouse embryonic fibroblasts (MEFs) for WT, CSE-/- and 3-MST-/-animals. Various signaling pathways were studied including the ubiquitin-proteasome pathway, autophagy-lysosomal pathways, antioxidant enzymes and mechanisms, the DNA damage response, mitochondrial function as well as key metabolic pathways. Preliminary findings show that in some signaling pathways there are significant differences between MEFs derived from CSE-/- vs 3-MST-/-. These reflect the effect of genetic deletion of CSE or 3-MST in cellular function and homeostasis, and consequently the different roles and contributions of these enzymes in the abovementioned signaling pathways.

Science

Health Sciences

hydrogen sulfide, signaling, CSE, 3-MST

Yes

2

Yes

143

93

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https://pergamos.lib.uoa.gr/uoa/dl/object/2931589