Unit:
Κατεύθυνση Αναπαραγωγική-Αναγεννητική ΙατρικήLibrary of the School of Health Sciences
Supervisors info:
Μάρα Σιμοπούλου, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Δέσποινα Μαυρογιάννη, Ε.ΔΙ.Π., Ιατρική Σχολή, ΕΚΠΑ
Σοφοκλής Σταύρου, Μαιευτήρας – Γυναικολόγος, Α΄ Μαιευτικής & Γυναικολογικής Κλινικής, Γ.Ν.Α. «ΑΛΕΞΑΝΔΡΑ»
Original Title:
H ανώμαλη γονιμοποίηση εμβρύων μετά από εφαρμογή της ICSI τεχνικής και η συσχέτισή της με παραμέτρους σπερμοδιαγράμματος: μια αναδρομική μελέτη παρατήρησης
Translated title:
Abnormal Fertilization in ICSI and association with abnormal semen parameters : A retrospective observational study on 1855 cases
Summary:
The Intracytoplasmic Sperm Injection (ICSI) technique is employed as the standard insemination strategy for managing cases of male infertility in the in vitro fertilization (IVF) laboratory. Nevertheless, it cannot be considered as the optimal practice, especially on the grounds of the abnormal presence of pronuclei (PNs) that may hinder the fertilization success. The aim of this study is to examine whether the presence of abnormal fertilization patterns- being apparent after ICSI technique- could be related with specific abnormal semen analysis parameters. This retrospective observational study involves 1855 fresh ICSI cycles, which were conducted in couples that were diagnosed with male factor infertility, based on World Health Organization criteria.
The study population was categorized based on the abnormal parameters identified after the semen analysis and their corresponding combinations. Contrarily, the control group included couples presenting with normal semen analysis. Normal fertilization was confirmed by the appearance of two PNs and two polar bodies. Various pathologies have been shown to affect the rate of 2PNs formation. An interesting finding is that no association was found between anomalies of semen analysis and 1PN or 3PNs formation rates. Moreover, patients presenting oligoasthenoteratozoospermia were observed with the lowest unsuccessful fertilization rate, whereas the normal group documented with the highest rate. Furthermore, both groups of oligoasthenozoospermia and oligoasthenoteratozoospermia were voiced with the lowest rates of embryos at the cleavage stage. The latter groups of patients combined with the oligoteratozoospermic ones also showed the lowest blastocyst formation rate. On top of that, only the groups of oligoasthenozoospermia and oligoasthenoteratozoospermia were correlated with reduced clinical pregnancy rates. It is also worth mentioning that, statistical adjustment for number of blastocysts did not affect the clinical pregnancy rates.
Our results indicate that the appearance of at least two abnormal semen analysis parameters, giving a great emphasis on oligozoospermia may exert a negative impact on normal fertilization, the cleavage and the blastocyst formation rates, as along with clinical pregnancy rates. It is of high importance the fact that when blastocyst is formed, only oligoasthenozoospermia and oligoasthenoteratozoospermia could affect the reproductive outcome. To conclude, the observation of two or more abnormal semen analysis parameters is vital for the decision-making process, providing information regarding embryo culture and the day of embryo transfer.
Main subject category:
Health Sciences
Keywords:
PNs, 1PN, 3PNs, ICSI, Male Infertility
File:
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MSc Thesis_ΚΑΡΑΝΤΖΑΛΗ ΕΛΕΝΗ.pdf
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