Association of GSTM1 polymorphism with male infertility and oxidative stress

Postgraduate Thesis uoadl:2933324 29 Read counter

Κατεύθυνση Αναπαραγωγική-Αναγεννητική Ιατρική
Library of the School of Health Sciences
Deposit date:
Voitse Anastasia
Supervisors info:
Δρακάκης Πέτρος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Μαυρογιάννη Δέσποινα, ΕΔΙΠ, Ιατρική Σχολή, ΕΚΠΑ
Σταύρος Σοφοκλής, Ακαδημαϊκός Υπότροφος, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Συσχέτιση του πολυμορφισμού GSTM1 με την ανδρική υπογονιμότητα και το οξειδωτικό στρες
Translated title:
Association of GSTM1 polymorphism with male infertility and oxidative stress
Approximately 7% of men worldwide are affected by male infertility, which contributes to 40-50% of all infertility cases. Basic sperm analysis remains the main procedure of diagnosing male infertility, although there is still doubt whether it provides accurate clinical outcomes. The test yields highly variable results from the same individual, inconsistent semen parameters among different observers, and no information about sperm dysfunctions at the molecular level. Thus we come to the conclusion that other, more accurate tests are essential for the diagnosis of male infertility.

In the present dissertation, we study the relationship between the GSTM1 polymorphism for oxidative stress and the parameters of the spermiodiagram, in order to create a map of genetic indicators for the most valid diagnosis of male infertility, in the greek population. This study shows that the presence of the GSTM1 polymorphism in the fertility group was correlated with fewer immobile sperm cells and the normal morphology of the sperm cells while in the infertility group there was significant correlation with age. In addition, we investigated the effect of oxidative potential with the MiOXSYS system and its relationship with sperm parameters. It was found that the two groups differed significantly when measuring oxidative potential, and that ORP affects sperm concentration/ml, total sperm count, type B motility and viscosity in the infertile male group.

Based on the above findings, it appears that GSTM1 polymorphism in the greek population would probably not be much of a useful indicator on its own, without ruling out that the use of other genetic markers related to oxidative stress may be a useful tool, although more studies should be performed. In addition, the measurement of oxidative stress can be included in daily clinical practice as it offers useful results for a more personalized treatment.
Main subject category:
Health Sciences
Male infertility, Oxidative stress
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