Unit:
Κατεύθυνση Φαρμακευτική Ανάλυση-Έλεγχος ΠοιότηταςLibrary of the School of Science
Supervisors info:
Ειρήνη Παντερή
Καθηγήτρια του τμήματος Φαρμακευτικής Χημείας στο Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Original Title:
Ανάπτυξη μεθόδου εκχύλισης προσρόφησης σε υφασμάτινο μέσο και αντίστροφης φάσης υγροχρωματογραφίας υψηλής απόδοσης με ανιχνευτή UV (FPSE-HPLC-UV) για την ανάλυση parabens σε ανθρώπινο ορό
Translated title:
Development of a Fabric Phase Sorptive Extraction High Performance Liquid Chromatographic Method with UV detection for the analysis of parabens in human serum
Summary:
Parabens are esters of p-hydroxy-benzoic acid and they are widely used as preservatives in medicines, cosmetics and food. In the late 2000s, these compounds began to raise concerns as they were found to have estrogenic action, and their detection in human breast tumors sparked a series of reports that cast doubt on their safety. These compounds are endocrine disruptors (EDCs), as they alter the function of the endocrine system. That is why it is necessary to study them further in biological samples.
The aim of this research was to develop and to evaluate a high-performance liquid chromatography method for the analysis of parabens and specifically of Methylparaben (MPB), Ethylparaben (EPB), Propylparaben (PPB), Iso-propylparaben (iPPB), Butylparaben (BPB) iso-butylparaben (iBPB) and benzylparaben (BzPB) in human serum. Fabric Phase Sorptive Extraction (FPSE), an innovative and promising technique was used to pre-treat the biological sample, which combines high selectivity and sensitivity. The ideal chromatographic method was developed as well as the optimal conditions that allowed the efficient separation of the analytes from matrix interferences. The optimal mobile and static phase and the appropriate detection conditions were selected. Fabric Phase Sorptive Extraction (FPSE) was adapted and optimized to achieve optimal analyte recovery and reduce interference from the matrix material.
Main subject category:
Science
Keywords:
HPLC, Bioanalysis, Human serum, Parabens, FPSE
