Unit:
Κατεύθυνση Αναπαραγωγική-Αναγεννητική ΙατρικήLibrary of the School of Health Sciences
Author:
Makri Christianna
Supervisors info:
Δημήτρης Λουτράδης, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ντόμαλη Αικατερίνη, Επίκουρη Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Δέσποινα Μαυρογιάννη, Βιολόγος, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Μελέτη της έκφρασης των γονιδίων PER1 και INCENP σε μορίδιο και βλαστοκύστη εμβρύου επίμυος
Translated title:
Study on the expression of PER1 and INCENP genes in morula and blastocyst stage of mouse embryo
Summary:
In mammals, the formation of the zygote becomes apparent after the coalescence of the female and the male pronucleus. Initially the fertilized ovum is divided into 2, 4 and 8 cells. By the early stage of eight cells, the shape of the blastomeres remains clear. The embryo is a loose structure with enough space between the cells. After the 3rd division the cells change behavior, tighten and form a sphere. The embryo at this stage is called a morula. When the morula enters the uterine cavity, fluid begins to penetrate through the zona pellucida into the intercellular space of the inner cell mass. The intercellular space begins to coalesce and eventually forms a cavity, the blastocoel. The embryo is characterized as a blastocyst and consists of 16 to 32 cells.
The aim of this dissertation is to study the expression of PER1 and INCENP proteins in the developmental stage of the morula and the blastocyst in mouse embryos.
PER1 protein is a product of the Per1 gene, which belongs to the largest category of "clock genes" that make up the basic structure of the circadian clock, which regulates metabolic and physiological rhythms in various organisms. The INCENP protein is one of the key subunits of the protein-passenger complex that includes Aurora-B protein as a key unit. The action of INCENP is necessary for the proper alignment of chromosomes during their separation at opposite poles during the cell cycle.
To study the expression of these proteins, the total RNA was isolated in the specific stages after the in-vitro culture of the embryos in conditions that simulate the environment of the uterus. Then, through the Real Time-PCR technique, the total expression of the proteins was calculated.
Main subject category:
Health Sciences
Keywords:
Mouse, Embryo, Morula, Blastocyst, PER1, INCENP
