Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Author:
Karagiannis Apostolos
Dissertation committee:
Μαυραγάνη Κλειώ, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Τσελένη-Μπαλαφούτα Σοφία, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Κουτσιλιέρης Μιχαήλ, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Κοτσιφάκη Ελένη, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Φιλίππου Αναστάσιος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Αρμακόλας Αθανάσιος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Χατζηγεωργίου Αντώνιος, Επίκουρος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Η έκφραση του ινσουλινομιμητικού αυξητικού παράγοντα στον καρκίνο του θυρεοειδούς
Translated title:
The expression of insulin like growth factor in thyroid cancer
Summary:
Background: Recent knowledge implicates a differential expression of the insulin-like growth factor-1 (IGF-1) mRNA splice variants (i.e., IGF-1Ea, IGF-1Eb and IGF-1Ec) in cancerous tissues, implying possible specific roles of the encoded IGF-1 protein isoforms in cancer biology. In particular, there is growing evidence that IGF-1Ec isoform may plays a distinct biological role in various types of cancer. The aim of the present study was to investigate whether IGF-1Ec isoform expression is associated with a particular type of thyroid cancer.
Materials and Methods: Formalin-fixed paraffin-embedded tissue specimens of different types of thyroid cancer from 92 patients were assessed for IGF-1Ec expression profile by immunohistochemistry. In addition, thyroid cancer biopsies of different TNM staging histological types were evaluated for mRNA expression of the IGF-1Ec transcript by real-time polymerase chain reaction (rt-PCR).
Results: From the total number of 92 samples, 2 were undifferentiated carcinomas, 10 medullary carcinomas, 4 hyperplasia of C-cell, 11 follicular carcinomas, 5 hurtle cell adenomas, 2 poor differentiated, 5 nodular hyperplasia, 1 lymphoma and 52 were papillary thyroid carcinomas. The age of the patients at the time of diagnosis or the tumor size did not affect significantly the IGF-1Ec expression. Among all types of cancer, IGF-1Ec was expressed in papillary differentiated thyroid carcinoma. Its expression/localization was mainly cytoplasmic and significantly positively associated with TNM staging and the presence of muscular and capsule cancerous invasion (p<0.05), while no differences were found regarding vascular or regional lymph node infiltration. Similarly, a differential profile was revealed regarding the mRNA expression of the IGF-1Ec transcript, which exhibited a higher expression in advanced papillary thyroid carcinoma.
Conclusion: The present data demonstrate that the expression of IGF-1Ec isoform in thyroid cancer is positively-associated with more advanced stages of papillary thyroid carcinoma.
Main subject category:
Health Sciences
Keywords:
Thyroid cancer, Igf-1, Papillary thyroid cancer, Igf-1ec, Biomarker
