Dissertation committee:
Γεώργιος Γκιόκας, Καθηγητής, Ιατρικής Σχολής, ΕΚΠΑ
Σταμάτιος Θεοχάρης, Καθηγητής, Ιατρικής Σχολής, ΕΚΠΑ
Νικόλαος Αρκαδόπουλος, Καθηγητής, Ιατρικής Σχολής, ΕΚΠΑ
Νικόλαος Δάφνιος, Καθηγητής, Ιατρικής Σχολής, ΕΚΠΑ
Ιωάννης Παπανικολάου, Αναπληρωτής Καθηγητής, Ιατρικής Σχολής, ΕΚΠΑ
Νικόλαος Δανιάς, Επίκουρος Καθηγητής, Ιατρικής Σχολής, ΕΚΠΑ
Νικόλαος Μιχαλόπουλος, Επίκουρος Καθηγητής, Ιατρικής Σχολής, ΕΚΠΑ
Summary:
Purpose: The purpose of the experiment is to evaluate whether antioxidant preconditioning with Deferoxamine can attenuate liver ischemia reperfusion injury associated with extended hepatectomy in swine.
Methods: Eighteen swine were randomly assigned to two groups: Deferoxamine (DFO) and Surgery Only (SO). The porcine in both groups were subjected to laparotomy, prolonged temporary occlusion of right and middle hepatic pedicles and subsequent left hepatectomy. DFO group received IV deferoxamine prior to induction of liver ischemia. Monitoring was performed for 6 hours and samples (Protein carbonyls, Thiobarbituric acid reactive substances, Histology, ALT, AST, Lactic acid and WBC) were drawn at 0, 60 and 360 mins.
Results: Protein carbonyls and Thiobarbituric acid reactive substances had significantly lower concentration and higher reduction rates in serum and liver tissue of DFO group. The histological examination of liver tissue showed less inflammation and necrosis in DFO group. Hepatic enzymes and lactic acid measurements showed higher reduction rate in DFO group by the end of the experiment.
Conclusions: This experimental study documents an early protective effect of deferoxamine administration in major hepatectomies against liver ischemia/reperfusion injury.
Keywords:
Deferoxamine, Ischemia, Reperfusion, Hepatectomy, Swine
