Faculty of Medicine
Library of the School of Health Sciences

2022-10-13

2022

Kyriakopoulos Konstantinos

Θόδωρος Λιακάκος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ευάγγελος Φελέκουρας, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Νικόλαος Νικητέας , Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Χαράλαμπος Χρέλιας , Αναπλ. Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ανδρέας Αλεξάνδρου, Αναπλ. Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Αλέξανδρος Χαραλαμπόπουλος , Αναπλ. Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ευάγγελος Μισσιακός, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ

Ο ρόλος των κυτταρικών υποδοχέων TLRs σε σηπτικό μοντέλο πυόμητρας μυών

Greek

Role of TLRS in a pyometra mouse model

Background: Pyometra leads to sepsis and multiple organ dysfunction syndrome (MODS). Toll like receptors (TLRs) recognize pathogens which can cause pyometra. The aim of this study was to investigate TLR-7 and -9 via the MYD88 pathway and the NFκB response in the uterus of a pyometra mice model before and after ovariohysterectomy as well as potential lung injury.
Materials and Methods: 200 female C57BL / 6J mice were randomly divided in groups (N=10/subgroup; SH1, SH2, SH3, SH7; P1, P2, P3, P7; 1RP1,1RP2, 1RP3, 1RP7; 2RP1, 2RP2, 2RP3, 2RP7; 3RP1, 3RP2, 3RP3, 3RP7) according to the day of euthanasia. Pathogens were administrated in the groups P and RP in order to induce pyometra.
Results: Alterations in blood, histopathology and qRT-PCT analysis before (P) and after ovariohysterectomy (RP) were observed. Significant correlations were also found between MYD88, NFκB and TLR-9 in P and RP groups in the lungs and in RP groups in the uterus, suggesting that the immune system responded via the TLR-9-MYD88 pathway.
Conclusions: This is the first report of immunohistochemical TLR-7 and -9 localization, and of TLR-7, -9, MYD88, and NFκB mRNA expression in the uterus causing lung injury in a pyometra mice model.

Health Sciences

Tlrs, Pyometra, Sepsis

No

0

Yes

544

210

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https://pergamos.lib.uoa.gr/uoa/dl/object/3236580