Unit:
Κατεύθυνση Αμπελουργία-Οινολογία και Αλκοολούχα ποτάLibrary of the School of Science
Author:
Kapetanakis Pantelis
Supervisors info:
1. Κατερίνα Μπινιάρη, Αναπληρώτρια Καθηγήτρια ΓΠΑ
2. Ερμόλαος Ιατρού, Καθηγητής ΕΚΠΑ
3. Μαριτίνα Σταυρακάκη, Επίκουρη Καθηγήτρια ΓΠΑ
Original Title:
Επίδραση αμπελοκομικών τεχνικών στην παρουσία ωχρατοξίνης Α σε σταφυλές
Translated title:
Επίδραση αμπελοκομικών τεχνικών στην παρουσία ωχρατοξίνης Α σε σταφυλές
Summary:
It is a well-known fact that ochratoxin A contaminates wine all around the world, and its consumption may significantly enhance human exposure to this toxin. More significantly, it has been shown to be nephrotoxic, hepatotoxic, teratogenic, and immunotoxic to numerous species of animals. Ochratoxin A contamination of grapes takes place in the field and is caused mainly by black Aspergilli, especially A. carbonarius. In this study, enzyme-linked immunosorbent assay (ELISA) was tested on must samples selected from grapes around Greece. The must samples that have been extracted from the grapes were neutralized using NaOH and diluted 2 times with 70% methanol. Then, they were used directly in the immunoassay and when the total procedure was completed; their absorbance is measured at 450nm. The samples were categorized and analyzed statistically in parameters: based on the grape variety, cultivation altitude, cultivation techniques, and interventions on a specific variety. Finally, according to these parameters, we conclude that OTA values, although they differ for each intervention, they do not deviate from the permissible limits making Greece a country with minor attacks from this mycotoxin.
Main subject category:
Science
Keywords:
ochratoxin A, OTA, must, ELISA, Aspergillus Carbonarius
Number of references:
122
File:
File access is restricted until 2026-03-14.
Διπλωματική Καπετανάκης.pdf
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File access is restricted until 2026-03-14.