Unit:
Κατεύθυνση Απομόνωση, Ανάπτυξη, Παραγωγή και Έλεγχος Βιοδραστικών Φυσικών ΠροϊόντωνLibrary of the School of Science
Author:
Mavrogiorgis Panagiotis
Supervisors info:
Γεωργία Βαλσαμή Καθηγήτρια, Τμήμα Φαρμακευτικής, ΕΚΠΑ,
Βασίλειος Ρούσσης Καθηγητής, Τμήμα Φαρμακευτικής, ΕΚΠΑ,
Ευσταθία Ιωάννου Αναπλ. Καθηγήτρια, Τμήμα Φαρμακευτικής ΕΚΠΑ
Original Title:
Ηλεκτροϊνοποιημένες μικρο-/νανοΐνες ως συστήματα ελεγχόμενης αποδέσμευσης του εχινοχρώματος Α
Translated title:
Electrospun micro-/nanofibers as controlled release systems of echinochrome A
Summary:
In the framework of the present thesis, the bioactive red pigment echinochrome A was isolated
from sea urchins of the genus Diadema, specimens of which were collected from Ag. Georgios
bay in Kastellorizo, at a depth of 1-5 m, in September 2021. Subsequently, micro-/nanofibrous
matrices based on inexpensive biodegradable polymers of hydrophobic and hydrophilic nature
were prepared using electrospinning, incorporating echinochrome A. The release rate of
echinochrome A from the developed micro-/nanofibrous matrices was evaluated in vitro, whereas
its permeability was investigated ex vivo using the small intestine of young rabbits as a
membrane. The morphological and physicochemical characterization of the electrospun patches
was performed using scanning electron microscopy (SEM), FT-IR spectroscopy,
thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Dissolution
assays using gastrointestinal-like fluids were performed to evaluate the release rate of
echinochrome A from the electrospun matrices. The permeability assay was performed in vertical
Franz type diffusion cells. The different formulations of the nonwoven fibrous matrices exhibited
variable release rates of echinochrome A, positively affecting the stability of the active substance
in aqueous media in all cases. In addition, the prepared micro-/nanofibrous scaffolds increased
the permeation of echinochrome A across the rabbit intestinal mucosa.
Main subject category:
Science
Keywords:
echinochrome A, micro-/nanofibers, electrospinning, dissolution, permeability
