Supervisors info:
Χήνου Ιωάννα Καθηγήτρια Τμήμα Φαρμακευτικής ΕΚΠΑ,
Αληγιάννης Νεκτάριος Καθηγητής Τμήμα Φαρμακευτικής ΕΚΠΑ,
Γκραίκου Κωνσταντία Μέλος Ε.ΔΙ.Π Τμήμα Φαρμακευτικής ΕΚΠΑ
Summary:
The subject of this study is the biological evaluation of aromatic medicinal plants of the
Prespa National Park, the chemical analysis of selected honey samples from the Prespa region
as well as the phytochemical study of the wild and in vitro produced Primula veris plant
species.Specifically, firstly, the area of the Prespa National Park is presented, with key points
being the legislative framework that protects the area, the geomorphology, the biodiversity,
and the main types of plants cultivated for their commercial importance.
The work is then divided into three chapters.
The 1st chapter deals withthe chemical analysis of the honey samples from stable bee-hives
of the area of Lake Prespas. In the first part of this chapter, there is a historical review of
honey and beekeeping, basic points about honey and the bee, the chemical composition and
biological actions of honey are presented.At the beginning of the second part (experimental
part) of the chapter, the principles of the method and the organology are presented, then the
results of the gyreological control, the determination of the quality control parameters, the
GC-MS analysis, the determination of the total phenolic load and the antioxidant capacity are
given in the honey samples from the areas of Ag. Achillios, Vrontero, Mikrolimni and Ag.
German of Lake Prespa. Finally, conclusions and prospects for exploiting the results are
drawn.
The 2nd chapter concerns the biological evaluation ofselected aromatic and medicinal plants
from the area of the Prespa National Park. The first part gathers all the data concerning their
morphological characteristics, their main metabolites, their biological actions and their
traditional uses, according to the existing bibliographic data. The second part describes the
extraction and analysis techniques, the principles of the method and the organology. Next,
the results of determining the total phenolic load and the antioxidant capacity of the extracts
are presented. Finally, the conclusions obtained from this study are presented.
The 3rd chapter concerns the phytochemical study of the wild species Primula veris subsp
veris from the area of the Prespa National Park and the in vitro produced plants of P.veris
from the "Hellenic Agricultural Organization Dimitra". The cultivated species is being studied
for the first time in Greece, as well as wild primulafram Lake Prespa. The first part
(introductory part) of the chapter focuses on the literature review of the geographical
distribution and botanical characteristics of the family, genus, and species.The drug chemistry
of the genus and its pharmacological actions are also presented. The second part includes the
description of the instruments and techniques of detection, separation and isolation, as well
as the determination of the structure of the metabolites by means of chromatographic and
spectroscopic methods. The results show in details the structural determination of all eleven
flavonoids identified. The methods for determining the total phenolic load and the
antioxidant capacity of the extracts are then described. Finally, the conclusions obtained from
the phytochemical study of the Primula veris subsp veris species from a wild and in vitro
produced population are presented.
Keywords:
Prespes, Honey, Medical plants, Primula veris, Phytochemistry
