Development, validation and applications of a GC-MS method for the determination of amphetamine type stimulants, synthetic cathinones and phenethylamines in whole blood.

Postgraduate Thesis uoadl:3329060 68 Read counter

Unit:
Κατεύθυνση Φαρμακευτική Ανάλυση-Έλεγχος Ποιότητας
Library of the School of Science
Deposit date:
2023-05-24
Year:
2023
Author:
Bouzoukas Christoforos
Supervisors info:
Ιωάννης Παπουτσής Αναπληρωτής Καθηγητής Τμήμα Φαρμακευτικής ΕΚΠΑ,
Ειρήνη Παντερή, Καθηγήτρια Τμήμα Φαρμακευτικής ΕΚΠΑ,
Ιωάννης Ντότσικας, Αναπληρωτής Καθηγητής Τμήμα Φαρμακευτικής ΕΚΠΑ
Original Title:
Ανάπτυξη, επικύρωση και εφαρμογές μεθόδου GC-MS για τον προσδιορισμό διεγερτικών τύπου αμφεταμίνης, συνθετικών καθινονών και φαιναιθυλαμινών σε ολικό αίμα
Languages:
Greek
Translated title:
Development, validation and applications of a GC-MS method for the determination of amphetamine type stimulants, synthetic cathinones and phenethylamines in whole blood.
Summary:
Amphetamine-type stimulants and mostly their newer substitutes; synthetic cathinones and phenethylamines pose a challenge to toxicology laboratories. Their extensive use and misuse has led to an increase of intoxications and fatal incidents. So the need for the development and validation of new analytical methods for their determination in biological samples is crucial for all toxicology laboratories worldwide for a better investigation of these cases.
The aim of this study was the determination of 9 amphetamine-type stimulants, 7 synthetic cathinones and 5 phenethylamines in whole blood using gas chromatography coupled with mass spectrometry.
The samples were pretreated by solid-phase extraction using a mixed mode column and then derivatized with pentafluoropropionic anhydride (PFPA). Chromatographic separation of the 21 analytes was achieved in less than 11 min. The method was then validated according to international guidelines for specificity, selectivity, range of linearity, limits of detection and quantification, precision, accuracy and recovery of the method. The LOD of the analytes ranged from 0.7-7 ng/mL and the values of the LOQ ranged from 2-20 ng/mL. The linearity of the 21 analytes depending on the substance were 2-200 ng/mL, 5-500 ng/mL, 10-500 ng/mL and 20-500 ng/mL with r2 values > 0.99. Extraction recovery rates were >80% for all analytes. Intra and inter day accuracy and precision of the method were within accepted limits. In addition, the method was also evaluated in urine samples on a single day of analysis for validation characteristics of whole blood.
The developed method was applied to post-mortem blood and urine samples of 40 forensic cases sent for toxicological investigation to the Department of Forensic Medicine and Toxicology, University of Athens. The results of the analyses revealed the presence of the stimulants amphetamine, pseudoephedrine, phenylpropanolamine, amphetamine, methamphetamine and MBDB.
Main subject category:
Science
Other subject categories:
Health Sciences
Keywords:
Amphetamine Type Stimulants, Synthetic Cathinones, Phenethylamines, Blood, Gas Chromatography-Mass Spectometry
Index:
Yes
Number of index pages:
3
Contains images:
Yes
Number of references:
182
Number of pages:
132
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