A study of the relationship of circulating amyloid precursor protein (APP), isoforms SAPPA and SAPPB, and heat stroke protein HSP-60 with cardiovascular indicators in postmenopsaus women

Doctoral Dissertation uoadl:3335944 1 Read counter

Faculty of Medicine
Library of the School of Health Sciences
Deposit date:
Soureti Anastasia
Dissertation committee:
Πανουλής Κωνσταντίνος, Καθηγητής Ιατρική Σχολή, ΕΚΠΑ
Λαμπρινουδάκη Ειρήνη, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Δρακούλης Νικόλαος, Αναπληρωτής Καθηγητής, Τμήμα Φαρμακευτικής, ΕΚΠΑ
Σοφία Καλανταρίδου, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Ελευθεριάδης Μακάριος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Καπαρός Γεώργιος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Σταυρούλα Πάσχου, Επίκουρη Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Μελέτη της συσχέτισης των κυκλοφορόντων πεπτιδίων amyloid precursor protein (APP), των ισόμορφων τους SAPPΑ και SAPPΒ και της πρωτεΐνης θερμικής καταπληξίας HSP-60 με καρδιαγγειακούς δείκτες των μετεμμηνοπαυσιακών γυναικών
Translated title:
A study of the relationship of circulating amyloid precursor protein (APP), isoforms SAPPA and SAPPB, and heat stroke protein HSP-60 with cardiovascular indicators in postmenopsaus women
Introduction: The hormonal changes of the menopausal transition are likely to be related with an increment of the individual cardiometabolic risk. Moreover, molecules like the amyloid-β (Αβ) and the heat shock protein 60 (HSP60) are involved in various pathophysiological alterations during the early stages of the atherosclerotic process, as per the results of in vitro studies but also the general population

Objective: The purpose of this study is to evaluate possible interactions between endothelial dysfunction and the following markers: amyloid-B markers and its isoforms as well as the Heat Shock Protein 60 index, in a sample of healthy postmenopausal women.

Material and method: This descriptive cross-sectional study evaluated healthy postmenopausal women examined at the Menopause Unit of Aretaieo Hospital, 2nd Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens. All women visiting the clinic for the first time were invited to participate in the study and underwent, prior to recruitment, a routine evaluation program including breast mammography, gynecological examination and Pap smear, as well as assessment of renal/thyroid/liver function. After applying the exclusion criteria, a total of 123 healthy women were selected to participate in this study.

In these women, serum total cholesterol, triglycerides, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol, lipoprotein and glucose levels, levels of HSP60, circulating peptides Amyloid Precursor Protein (APP) and their isoforms sAPPα and sAPPβ, insulin, FSH, estradiol, SHBG, testosterone and dehydroepiandrosterone levels and flow-mediated dilatation (FMD), wave been
assesed. Sonographical assessment included the estimation of the flow-mediation dilation (FMD) of the brachial artery.

Results: The results of this study support a significant positive association between FMD and HSP60 values, after adjustment for cardiovascular risk factors. Τhis association became non-significant after additional control for insulin resistance (HOMA-IR) as well as for levels of circulating androgens. Women with higher FSH values had higher HSP60 levels, independent of traditional cardiovascular risk factors. Adding other hormonal agents such as testosterone or DHEAS to the equation results in the loss of this balance, resulting in vascular lesions. Levels of Aβ or the Aβ-isoforms were not associated with circulating levels of HSP60 or values of FMD.

Conclusion: We observed that endothelial function in postmenopausal women is positively related to HSP60 levels. This association appears to be mediated by levels of circulating androgens. On the contrary, the levels of Aβ molecules or the Aβ-isoforms are not associated with values of FMD or the circulating levels of HSP60. More longitudinal studies are required to confirm the significance of our findings, in this group of women with heightened cardiovascular risk.
Main subject category:
Health Sciences
Menopause, Amyloids, HSP60, Cardiovascular Indicators
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