Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Dissertation committee:
Ανέστης Χαραλαμπόπουλος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ευάγγελος Μισιακός, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ζάβρας Νικόλαος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Eμμανουήλ Πικουλής, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Παύλος Πατάπης, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Κωνσταντίνος Νάστος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Διονύσιος Δελλαπόρτας, Επίκουρος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Η επίδραση του ηπατοκυτταρικού αυξητικού παράγοντα στην προσαρμογή του λεπτού εντέρου μετά από εκτεταμένη εντερεκτομή - πειραματική μελέτη σε επίμυες
Translated title:
The effect of hepatocyte growth factor on intestinal adaption in an experimental model of short bowel syndrome
Summary:
Purpose
Nowadays, the standard therapy for patients with short bowel syndrome is parenteral nutrition (PN). Various growth factors have been tested in order to achieve weaning from prolonged PN administration. We evaluated the effect of hepatocyte growth factor (HGF) on structural intestinal adaptation and cell proliferation in a rat model of SBS.
Methods
Thirty Sprague-Dawley rats were divided into three groups; group A rats (sham) underwent bowel transection, group B rats underwent a 75% bowel resection and group C rats underwent the same procedure but were treated postoperatively with HGF. Histopathologic parameters of intestinal adaptation were determined, while a microarray and a rt-PCR analyses of ileal RNA was also performed.
Results
Treatment with HGF resulted in significant increase in body weight, while the jejunal and ileal villus height and crypt depth were increased in HGF rats (36%, P<0.05 and 27%, P<0.05 respectively). Enterocyte proliferation was also significantly increased in HGF rats (21% P<0.05). Microarray and quantitative rt-PCR analyses showed that the genes hgfac, rac 1, cdc42 and akt 1 were more than two-fold up-regulated after HGF treatment.
Conclusion
HGF emerges as a growth factor that enhances intestinal adaptation. The future use of HGF may potentially reduce the requirement for PN in SBS patients.
Main subject category:
Health Sciences
Keywords:
short bowel syndrome, Hepatocyte growth factor, Intestinal adaptation, Cell proliferation
Number of references:
124
File:
File access is restricted until 2025-07-29.
HGFSBSBOOK.pdf
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File access is restricted until 2025-07-29.
