Unit:
Department of ChemistryLibrary of the School of Science
Dissertation committee:
1. Ερμόλαος Ιατρού, Καθηγητής, ΕΚΠΑ
2. Παναγιώτης Αργείτης, Διευθυντής Ερευνών, ΕΚΕΦΕ «Δημόκριτος»
3. Κακαμπάκος Σωτήριος, Διευθυντής Ερευνών, ΕΚΕΦΕ «Δημόκριτος»
4. Πατρίνα Παρασκευοπούλου, Αν. Καθηγήτρια, ΕΚΠΑ
5. Μαρίνος Πιτσικάλης, Καθηγητής, ΕΚΠΑ
6. Παναγιώτα Πέτρου, Διευθύντρια Ερευνών, ΕΚΕΦΕ «Δημόκριτος»
7. Μαργαρίτα Χατζηχρηστίδη, Αν. Καθηγήτρια, ΕΚΠΑ
Original Title:
Κατασκευή Βιο-Λειτουργικών Επιφανειών με Χημεία Click και Τεχνικές Φωτολιθογραφίας
Translated title:
FABRICATION OF BIO-FUNCTIONAL SURFACES USING CLICK CHEMISTRY AND PHOTOLITHOGRAPHY TECHNIQUES
Summary:
The aim of this PhD thesis is the modification of surfaces for biological applications such as the development of microarrays of biomolecules, using a new lithography technique called multi-cycle lithography in combination with «click» chemistry for the covalent attachment of biomolecules to a suitable substrate. In the present work dibenzylcyclooctyne and azide were used as reactive «click» groups. Also, azide photochemistry was studied as an alternative method for the successful immobilization of molecules.
Processes were developed for attaching biomolecules to a substrate modified with an epoxy resin (EPR) film and to self-assembled monolayers SAMs bearing side groups with an epoxy ring or an amino group. Initially, covalent biotin derivatives or other molecules bearing groups suitable for subsequent «click» chemistry were attached to the modified surfaces. In each case, the successful immobilization of the molecules through «click» chemistry on the modified surfaces by reaction with a suitable fluorescent substance bearing a «click» chemistry group was studied. Azide photochemistry was used, exploiting their characteristic property that upon exposure to deep UV (254 nm) N2 is released producing nitrenes, which then react with the double bond of the polymer chains acting as cross-linkers. On the other hand, «click» chemistry was chosen as «click» reactions are characterized by very high yields, simplicity in implementation and are stereospecific.
In addition, a lithographic scheme for biomolecule arrangement based on the use of photoresist that can be used in biocompatible conditions was studied. In this work, the possibility of using and extending the same approach for the development of microarrays from different proteins was investigated.
Main subject category:
Science
Keywords:
click chemistry, surface modification, SAMS, azide photochemistry, epoxy resist, multi-cycle lithography
Number of index pages:
15
Number of references:
246
File:
File access is restricted until 2025-10-01.
VRETTOU PHD.pdf
5 MB
File access is restricted until 2025-10-01.
