Unit:
Κατεύθυνση Παιδιατρική ΛοιμωξιολογίαLibrary of the School of Health Sciences
Supervisors info:
Βασιλική Παπαευαγγέλου, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ, Επιβλέπουσα
Δέσποινα Μπριάνα, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Άννα Παπαδοπούλου, ΕΔΙΠ, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Προσδιορισμός της συγκέντρωσης των σιαλικών οξέων και των βλεννινών στο μητρικό γάλα πριν και μετά την παστερίωση.
Translated title:
Measurment of sialic acid and mucins concentrations in human milk before and after holder pasteurization
Summary:
Introduction: Human milk not only supplies the nutritional needs of the newborn, but it also possesses anti-inflammatory, anti-microbial, and immunomodulatory properties, which play a vital role in infant development and protection against pathogens. Bioactive substances, such as sialic acids and mucins, confer these properties to human milk. This thesis investigates the alteration of sialic acids and mucins concentrations in preterm human milk before and after Holder Pasteurization.
Methods: Human milk samples were collected at approximately the 5th and the 15th day after delivery, from 31 mothers, with gestational age up to 36 weeks. Half of each sample underwent Holder Pasteurization (ΗP), while the other half was stored as ‘fresh-frozen milk’ at -800C (FF). The quantitative assessment of mucins and free and total sialic acids was determined using ELISA and chromatography. The patient characteristics of the mother and the neonate were collected at approximately the 5th day after delivery and were used for statistical analysis. The statistical analysis was performed using SPSS.
Results: 31 mothers participated in this study, with a median age of 34 years and a median gestational age of 34 weeks, who provided a breast milk sample at approximately the 5th day after delivery, while 14 of them provided an additional breast milk sample at approximately the 15th day after delivery. 71%, of the mothers who participated underwent a caesarean section. Free and total sialic acids concentrations, did not differ post pasteurization, neither on samples collected on day 5 or 15 post-birth. The concentration of total sialic acids was statistically significantly higher in fresh frozen milk samples (p-value 0.011) and pasteurized milk samples (p-value 0.022) collected around the 5th day after parturition compared to those collected around the 15th day. However, the difference in the concentration of free sialic acids was not statistically significant in fresh frozen milk samples (p-value 0.975) and the samples of pasteurized milk (p-value 0.925) collected around the 5th day after delivery compared to those collected around the 15th day. It should be noted that the mucins detection method failed.
Conclusions: Pasteurization of breast milk does not appear to affect the concentration of total and free sialic acids. However, the concentration of TSA decreases statistically significantly around the 15th day after delivery, while the concentration of FSA does not seem to change in a statistically significant manner.
Conclusions: Pasteurization of breast milk does not appear to affect the concentration of total and free sialic acids. However, the concentration of TSA decreases statistically significantly around the 15th day after delivery, a finding supported by literature. The concentration of FSA does not seem to change in a statistically significant manner.
Main subject category:
Health Sciences
Keywords:
Human milk, Sialic acid, Holder pasteurization, Neonates, Mucins
Number of references:
123
File:
File access is restricted until 2025-03-28.
ΔΙΠΛΩΜΑΤΙΚΗ FINAL.pdf
11 MB
File access is restricted until 2025-03-28.
