Unit:
Κατεύθυνση Θρόμβωση-Αιμορραγία-Ιατρική των μεταγγίσεωνLibrary of the School of Health Sciences
Author:
Nikitiadis Konstantinos
Supervisors info:
Πολίτου Μαριάννα, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Μερκούρη Ευφροσύνη, Επιστημονικός Συνεργάτης Π.Μ.Σ. “Θρόμβωση – Αιμορραγία –
Ιατρική των μεταγγίσεων”, Ιατρική Σχολή, ΕΚΠΑ
Σταμούλης Κωνσταντίνος, Επιστημονικός Συνεργάτης Π.Μ.Σ. “Θρόμβωση – Αιμορραγία –
Ιατρική των μεταγγίσεων”, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Η χρήση της θρομβοελαστομετρίας ROTEM ως δείκτης ποιότητας του φρέσκου κατεψυγμένου πλάσματος (FFP)
Translated title:
The use of ROTEM thromboelastometry as a quality indicator of fresh frozen plasma (FFP)
Summary:
Introduction – Aim: Factor VIII (FVIII) levels are used as a marker of fresh frozen plasma (FFP) quality, but without measuring other coagulation factors. The aim of the present study is, in addition to the evaluation of FVIII, to evaluate the overall hemostatic function of plasma using rotational thromboelastometry (ROTEM).
Materials and Method: Plasma from 20 healthy adult whole blood donors was studied at 5 time points (time points): TP0: Blood collection (CPD, Macopharma), TP1: FFP production (3000 rpm 15 min) within 4 h of collection, TP2: Thawing 1 month after storage at -28°C in a water bath (37°C), TP3: 24h after thawing and storage at 4°C, TP4: 48h after thawing and storage at 4°C. FVIII and FV factors were assessed at all time points (BCS XP System, Siemens, Chromogenic assay), and using the ROTEM rotational thromboelastometry, the EXTEM (with thromboplastin as activator) and NATEM (calcium as single activator) assays were performed.
Results: FV and FVIII showed a decreasing trend from time to time, with FV remaining stable from the time of thawing onwards, in contrast to FVII, which continued to decrease, scoring levels that did not meet quality standards for factor VIII. Clotting time (CT) and Clot Formation Time (CFT) showed prolongation of increased statistical significance from blood collection to FFP production, while no significant changes were documented at the other time points. Likewise, Maximum Clot Firmness (MCF) showed a significant decrease from blood collection to FFP production and then it was stabilized.
Conclusion: These data suggest that levels of unstable factor VIII decrease progressively and to a greater extent than those of factor V. The majority of thromboelastometry parameters appear to be significantly affected by the FFP production process, whereas storage conditions do not show significant variations between the time points studied. Thus, despite the reduction of unstable factors VIII and V, the formation time, stability and clot strength are maintained at a good level from the time of thawing onwards reflecting at the same time a good preservation of the activity of clotting factors in plasma produced from whole blood.
Main subject category:
Health Sciences
Keywords:
Blood Donation, Quality Control, Blood Products, Fresh Frozen Plasma, Thromboelastometry
Number of references:
204
File:
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Nikitiadis_Konstantinos_Msc.pdf
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