Κατεύθυνση Κλινική Βιοχημεία - Μοριακή Διαγνωστική
Library of the School of Science

2024-05-13

2024

Nizami Aikaterini

Ανδρέας Σκορίλας, Καθηγητής, Τμήμα Βιολογίας, ΕΚΠΑ,
Χρήστος Κοντός, Επίκουρος Καθηγητής, Τμήμα Βιολογίας, ΕΚΠΑ,
Μαργαρίτης Αυγέρης, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ

Ανάλυση και μελέτη της έκφρασης μικρών μη κωδικών μορίων RNA που στοχεύουν γονίδια των καλλικρεϊνών σε καρκινικά κύτταρα

Greek

Ανάλυση και μελέτη της έκφρασης μικρών μη κωδικών μορίων RNA που στοχεύουν γονίδια των καλλικρεϊνών σε καρκινικά κύτταρα

Cancer is a disease that has been of great concern to the scientific community in recent decades given its high prevalence and the high mortality of cancer patients. There is a great need to find ways of both prevention and treatment. Changes in gene expression associated with cancer are caused by dysfunctions of various regulators, including non-coding RNAs whose role is to repress or reduce gene expression at the transcriptional level. Non-coding RNAs have been associated with many proteins, including kallikreins. These molecules are serine proteases with trypsin-like or chymotrypsin-like specificity and are widely believed to be involved in pathways that regulate kidney function, skin exfoliation, synaptic neural plasticity, brain function, tooth enamel formation as well as sperm fluidity. Kallikrein 3 or PSA is expressed in the prostate gland and is responsible for sperm fluidity. PSA has great diagnostic value in prostate cancer. This thesis aimed to identify novel non-coding RNA molecules through bioinformatics analysis of data obtained from next-generation sequencing, to study their expression in human cancer cell lines and to study them as potential targets of kallikrein genes. Specifically, .FASTQ files, derived from NGS experiments in the laboratory, were submitted to the miRDeep* program, indicating 32 novel non-coding RNA molecules. To study them further and select those of interest we used the Blast, miRBase and miRDB databases from which we selected 8 according to specific criteria. Finally, quantitative real-time PCR (qPCR) was performed to study the expression levels of the 8 new candidate sequences. The experimental procedure revealed that the BankIt2351829 molecule is overexpressed in most cell lines and through miRDB seems to have a high complementarity with KLK3. This finding is extremely important as it is an indication that it may be involved in prostate cancer.

Science

small non-coding RNAs, kallikreins

No

0

Yes

138

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File access is restricted until 2024-11-16.

https://pergamos.lib.uoa.gr/uoa/dl/object/3398497