Unit:
Κατεύθυνση Αναλυτική Χημεία-Διασφάλιση ποιότηταςLibrary of the School of Science
Author:
Preza-Mayo-Kataki Dafni-Angelika
Supervisors info:
Νικόλαος Θωμαΐδης, Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Ευάγγελος Γκίκας, Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Μαριλένα Δασενάκη, Επίκουρη Καθηγήτρια, Τμήμα Χημείας, ΕΚΠΑ
Original Title:
Extended chemical characterization of selected Greek bee products using cutting-edge mass spectrometry techniques.
Translated title:
Extended chemical characterization of selected Greek bee products using cutting-edge mass spectrometry techniques.
Summary:
In this master thesis, the bioactive content of several Greek bee products was studied
and characterized, with emphasis on the phenolic fraction. To achieve this goal the
cutting-edge UHPLC-VIP-HESI-TIMS-QTOF-MS analytical technique was used. This is
the first time that this innovative technique has been employed for the determination of
phenolic compounds in bee products. Initially, the analytical method was validated for 52
analytes using standard solutions of phenolic compounds and was found to be fit for
purpose. Following validation, the method was used for the analysis of royal jelly, bee
pollen, and propolis samples of Greek origin, leading to the identification and
quantification of 24, 43, and 48 phenolic compounds respectively, via target screening. In
addition, phenolic compounds that have been previously reported in bee products, based
on a thorough literature review, were recorded and a suspect list of 25 compounds was
created. Using this suspect list 14 phenolic compounds in royal jelly, 16 in bee pollen,
and 18 in propolis were identified, leading to an in-depth characterization of their phenolic profiles.
Main subject category:
Science
Keywords:
Liquid chromatography, trapped ion mobility spectrometry, validation, bee products, phenolic compounds
File:
File access is restricted until 2027-06-21.
MASTER THESIS FINAL PREZA-MAYO-KATAKI.pdf
4 MB
File access is restricted until 2027-06-21.
