Κατεύθυνση Αναλυτική Χημεία-Διασφάλιση ποιότητας
Library of the School of Science

2024-06-23

2024

Panagopoulou Dimitra

Νικόλαος Θωμαΐδης, Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ (Επιβλέπων),
Ευάγγελος Γκίκας, Καθηγητής Τμήμα Χημείας ΕΚΠΑ
Μαριλένα Δασενάκη, Επικ. Καθηγήτρια Τμήμα Χημείας ΕΚΠΑ

Identification of authenticity markers in food of animal origin with High Resolution Mass Spectrometry

English

Identification of authenticity markers in food of animal origin with High Resolution Mass Spectrometry

Food of animal origin (fish and seafood, meat, milk and dairy products) plays a
significant role in human nutrition, being the main source of all necessary
nutrients. Focusing on meat and its products, are of great importance for both
the consumers and global economy due to their high commercial and nutritional
value. However, meat products are often being subjected to adulteration
practices. The most common illegal practices include the addition of different
animal species (e.g. horse and pork), mislabeling of the geographical origin or
mislabeling of the feeding practice of the animal. For this purpose, numerous
analytical methodologies have been developed and applied in order to offer
realistic solutions to the food industry. However, there is an imperative need to
improve the established methodologies and provide holistic workflows to
evaluate the origin of the food product.
During the last decades, High Resolution Mass Spectrometry (HMRS)
represents a significant analytical technique to address authenticity issues and
provide reliable results. Combined with metabolomics, an emerging omics based technique, comprises a promising approach for meat authenticity
investigation. The aim of the present master thesis is the development of a
holistic workflow to investigate authenticity of different animal muscle tissues,
exploiting the metabolite content. For this purpose, Reversed Phase Liquid
Chromatography (RPLC) and Hydrophilic Interaction Chromatography (HILIC)
coupled to Quadruple Time-of-Flight Mass Spectrometry (LC-QTOFMS)
operated in positive ion mode were combined in order to reach the maximum
metabolites coverage. Applying a target screening approach through the
exploitation of in-house databases, more than 90 metabolites were detected
and identified in each chromatography, belonging to the categories such as
amino acids and their derivatives, organic acids, fatty acids, vitamins etc. In
combination with chemometrics (PCA and PLS-DA), discrimination of samples
according to animal origin, animal muscle tissue type and feeding practice was
achieved, while potential authenticity markers were investigated and identified.

Science

Food authenticity, meat, metabolomics, LC-QTOFMS, target screening

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https://pergamos.lib.uoa.gr/uoa/dl/object/3401695